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[
1
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T
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T
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23
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No
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3
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J
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20
25
:
7
03
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7
15
704
leak
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ce
o
f
m
icr
o
o
r
g
an
i
s
m
ac
ti
v
it
y
alo
n
g
w
ater
d
is
tr
ib
u
tio
n
p
ip
elin
e
s
y
s
te
m
s
ar
e
m
ain
l
y
ca
u
s
ed
b
y
b
io
f
il
m
s
f
o
r
m
atio
n
o
n
i
n
ter
n
al
p
ip
eli
n
e
s
u
r
f
ac
es,
lead
in
g
th
e
t
h
e
s
u
b
s
ta
n
tial
g
r
o
w
t
h
o
f
m
icr
o
b
es
an
d
r
es
u
lted
i
n
r
ec
o
n
ta
m
i
n
an
tio
n
e
v
e
n
ts
[
5
]
.
Fu
r
th
er
m
o
r
e,
m
icr
o
b
es
wi
th
in
b
io
f
il
m
s
ca
n
also
d
ev
elo
p
ed
d
u
r
in
g
th
e
i
n
s
tallatio
n
p
r
o
ce
s
s
o
f
p
ip
e
s
u
r
f
ac
es.
C
er
tain
p
ath
o
g
en
i
c
m
icr
o
o
r
g
an
i
s
m
s
t
y
p
ica
ll
y
e
x
i
s
t
as
f
r
ee
-
f
lo
atin
g
s
i
n
g
le
ce
lls
in
p
lan
k
to
n
ic
f
o
r
m
w
it
h
i
n
th
e
W
DS
.
Ho
w
ev
er
,
u
p
o
n
attac
h
i
n
g
to
p
ip
elin
e
s
u
r
f
ac
es,
th
e
y
f
o
r
m
m
a
tr
ices
a
n
d
estab
lis
h
co
lo
n
ies.
T
h
ese
co
lo
n
ies
m
atu
r
e
w
it
h
i
n
th
e
b
io
f
il
m
m
atr
ix
,
cr
ea
ti
n
g
a
p
r
o
tectiv
e
s
h
ield
t
h
at
m
ak
e
s
d
etec
tin
g
th
e
s
e
m
icr
o
o
r
g
an
is
m
s
ch
allen
g
i
n
g
.
Ov
er
ti
m
e,
b
io
f
il
m
s
d
is
p
er
s
e
in
to
p
o
tab
le
w
ater
w
it
h
in
th
e
d
is
tr
ib
u
tio
n
s
y
s
te
m
,
u
lti
m
atel
y
r
ea
ch
i
n
g
co
n
s
u
m
er
s
.
I
n
ad
d
itio
n
to
th
e
f
ac
to
r
s
m
e
n
tio
n
ed
,
v
ar
i
o
u
s
o
t
h
er
cir
cu
m
s
tan
ce
s
co
n
tr
ib
u
te
to
w
ater
d
eter
io
r
atio
n
,
in
clu
d
i
n
g
ag
in
g
in
f
r
astru
c
tu
r
e,
s
u
r
f
ac
e
m
ater
i
als,
h
i
s
to
r
ical
b
r
ea
k
a
g
es,
o
p
er
atio
n
al
p
ar
a
m
eter
s
,
an
d
w
ater
ag
e.
R
esear
ch
in
d
i
ca
tes
d
is
ti
n
g
u
i
s
h
in
g
d
i
f
f
er
e
n
c
e
b
ac
ter
ial
s
p
ec
ies
s
u
r
v
i
v
i
n
g
i
n
w
ater
ec
o
s
y
s
te
m
s
ar
e
ex
tr
e
m
el
y
ti
m
e
co
n
s
u
m
i
n
g
w
h
ic
h
p
o
s
es
c
h
alle
n
g
es
an
d
in
v
asi
v
e
m
ea
s
u
r
es
[
6
]
.
I
n
ad
d
itio
n
,
s
e
v
er
al
d
is
tr
ib
u
tio
n
to
lp
o
lo
g
ie
s
an
d
co
m
p
o
n
e
n
t
s
in
teg
r
itie
s
,
s
u
ch
as
p
ip
es
m
ater
ial,
s
to
r
ag
e
f
ac
ilit
ies,
p
u
m
p
in
g
s
tatio
n
s
,
v
al
v
es
a
n
d
f
itti
n
g
s
,
u
n
i
n
te
n
tio
n
all
y
co
u
ld
s
er
v
e
as
id
ea
l
r
eso
u
r
ce
s
f
o
r
m
icr
o
b
ial
p
r
o
d
u
ctiv
it
y
.
P
r
ev
io
u
s
s
tu
d
ie
s
h
av
e
o
b
s
er
v
ed
s
u
b
s
tan
tial
b
ac
ter
ial
g
r
o
w
t
h
o
n
p
ip
elin
e
s
u
r
f
ac
e
s
,
in
clu
d
in
g
ir
o
n
an
d
p
last
ic
p
o
ly
v
i
n
y
l
c
h
lo
r
id
e
(
P
VC
)
p
ip
es
[
7
]
.
H
o
w
ev
e
r
,
i
r
o
n
p
i
p
e
l
in
es
a
p
p
e
a
r
t
o
b
e
p
a
r
t
i
c
u
l
a
r
ly
c
o
n
d
u
c
iv
e
to
m
icr
o
b
ial
ac
tiv
it
y
w
it
h
i
n
W
DS.
Mic
r
o
b
e
s
’
ab
ilit
y
to
d
o
m
in
ate
t
h
e
co
r
r
o
s
io
n
of
m
etals
li
k
e
ir
o
n
,
co
p
p
er
,
an
d
alu
m
in
u
m
,
t
y
p
icall
y
co
n
s
id
er
ed
co
r
r
o
s
io
n
-
r
e
s
is
ta
n
t,
p
o
s
es
a
g
en
u
i
n
e
t
h
r
ea
t
to
p
o
tab
le
w
ater
q
u
al
it
y
[
8
]
.
On
to
p
o
f
t
h
at
,
m
icr
o
b
es
th
at
s
u
r
v
i
v
ed
in
lo
w
-
o
x
id
ized
p
ip
elin
e
e
n
v
ir
o
n
m
en
ts
ca
n
ad
ap
t
to
ex
tr
e
m
e
c
o
n
d
itio
n
s
b
y
alter
i
n
g
th
eir
b
ac
ter
ial
m
o
r
p
h
o
lo
g
ie
s
,
in
clu
d
i
n
g
s
u
r
v
i
v
al
g
r
o
w
t
h
,
s
p
e
cies d
iv
is
io
n
,
an
d
s
h
ap
e
-
s
h
if
t
i
n
g
ab
ilit
ies.
W
i
t
h
a
n
o
t
a
b
l
e
a
b
s
e
n
c
e
o
f
r
e
s
ea
r
c
h
in
v
e
s
t
ig
a
tin
g
m
ic
r
o
b
i
a
l
c
o
n
t
am
in
a
n
t
s
,
f
u
r
th
e
r
a
c
ti
o
n
p
a
r
t
ic
u
l
a
r
ly
i
n
m
o
n
i
t
o
r
in
g
w
at
e
r
q
u
a
l
ity
a
t
p
o
i
n
t
-
of
-
u
s
e
(
PO
U
s
)
d
is
t
r
i
b
u
t
i
o
n
s
y
s
tem
s
is
n
e
c
es
s
a
r
y
.
T
h
e
ce
n
tr
a
l
p
r
o
b
l
em
r
el
a
t
e
d
t
o
w
at
e
r
d
e
t
e
r
i
o
r
a
t
i
o
n
i
s
r
e
c
o
n
t
am
in
a
t
i
o
n
o
c
c
u
r
r
en
ce
s
in
d
o
m
es
t
ic
w
a
t
e
r
s
y
s
t
em
s
at
h
o
u
s
e
h
o
l
d
o
r
r
e
s
i
d
en
t
i
al
PO
U
s
[
9
]
.
T
h
e
e
v
a
lu
a
t
i
o
n
o
f
w
a
te
r
q
u
a
li
ty
,
em
p
h
as
i
zi
n
g
id
e
n
t
if
i
c
a
ti
o
n
o
f
m
i
c
r
o
b
i
a
l
c
o
n
t
am
in
an
ts
i
n
w
at
e
r
s
y
s
tem
,
a
t
n
a
t
i
o
n
a
l
l
ev
e
l
is
s
t
i
ll
a
t
i
ts
in
f
an
cy
[
1
0
]
,
[
1
1
]
.
S
ev
e
r
a
l
l
im
it
at
i
o
n
s
o
f
r
e
c
en
t
w
a
t
e
r
q
u
a
li
ty
m
o
n
i
t
o
r
i
n
g
t
e
c
h
n
i
q
u
es
,
s
u
c
h
as
th
e
cu
l
tu
r
e
-
d
e
p
en
d
en
t
a
p
p
r
o
a
ch
an
d
R
i
b
o
n
u
c
l
e
i
c
A
c
i
d
(
R
NA
)
/
D
eo
x
y
r
i
b
o
n
u
cl
e
i
c
A
c
i
d
(
D
NA
)
ex
t
r
a
c
t
i
o
n
,
a
r
e
c
o
m
p
l
ex
,
t
im
e
-
c
o
n
s
u
m
in
g
,
c
o
s
t
ly
,
an
d
la
b
o
r
i
o
u
s
[
1
2
]
.
No
tab
l
y
,
t
h
ese
t
r
ad
itio
n
al
m
et
h
o
d
s
t
y
p
icall
y
an
a
l
y
ze
d
ata
in
s
p
ec
i
f
ied
lab
o
r
a
to
r
y
e
n
v
ir
o
n
m
e
n
ts
,
co
n
s
tr
ain
in
g
t
h
e
ab
ili
t
y
f
o
r
r
ea
l
-
ti
m
e
m
o
n
ito
r
in
g
.
Hen
ce
,
th
e
y
h
i
n
d
er
th
e
d
ev
elo
p
m
e
n
t
o
f
an
e
f
f
ec
tiv
e
m
o
n
ito
r
in
g
s
tr
ateg
y
f
o
r
d
etec
tin
g
co
n
t
a
m
i
n
an
t
s
at
P
OU
.
T
h
er
ef
o
r
e,
th
er
e
is
a
p
r
es
s
in
g
n
ee
d
to
u
n
d
er
s
ta
n
d
b
ac
ter
ial
co
m
m
u
n
it
y
p
r
esen
ce
p
atter
n
s
i
n
b
u
i
ld
in
g
W
DS
a
n
d
s
tag
n
a
n
t
tap
w
ater
u
n
d
er
v
ar
i
o
u
s
p
ip
elin
e
co
n
d
itio
n
s
.
Mo
s
t
w
ater
tr
ea
t
m
e
n
t
p
lan
t
s
an
d
u
t
ilit
ies
g
e
n
er
all
y
u
s
e
f
ae
ca
l
in
d
icato
r
s
as
a
s
ig
n
o
f
co
m
p
r
o
m
is
ed
w
ater
ec
o
s
y
s
te
m
s
.
Fo
r
in
s
tan
ce
,
th
e
p
r
esen
ce
o
f
E
.
co
li
ex
ce
ed
in
g
s
tan
d
ar
d
co
n
ce
n
tr
at
io
n
le
v
el
i
n
d
icate
s
p
o
s
s
ib
le
h
u
m
a
n
o
r
a
n
i
m
al
w
as
te
in
tr
u
s
io
n
w
it
h
i
n
d
is
tr
ib
u
tio
n
s
y
s
te
m
s
[
1
3
]
.
I
n
d
icato
r
o
r
g
an
is
m
s
,
s
u
ch
as
to
tal
co
lif
o
r
m
s
,
ar
e
f
r
e
q
u
en
tl
y
e
m
p
lo
y
ed
in
v
ar
io
u
s
w
ater
m
o
n
ito
r
in
g
m
et
h
o
d
s
.
Ho
w
ev
er
,
an
in
s
ig
n
if
ica
n
t
co
r
r
elatio
n
ex
is
ts
b
et
wee
n
th
ese
i
n
d
icato
r
s
an
d
th
e
ty
p
e
o
f
p
ath
o
g
e
n
ic
s
p
ec
ies
b
ec
au
s
e
t
h
e
ab
s
e
n
c
e
o
f
i
n
d
icato
r
s
i
n
w
ater
d
o
es
n
o
t
g
u
ar
an
tee
th
e
ab
s
e
n
ce
o
f
p
ath
o
g
en
ic
m
icr
o
o
r
g
an
i
s
m
s
.
As
d
escr
ib
ed
a
b
o
v
e,
m
a
n
y
m
o
n
ito
r
in
g
tec
h
n
iq
u
es
f
o
r
d
etec
tin
g
m
icr
o
b
io
lo
g
ical
co
n
ta
m
in
an
ts
ar
e
o
f
ten
c
u
m
b
er
s
o
m
e
a
n
d
co
m
p
l
ex
,
r
eq
u
ir
in
g
f
u
r
t
h
er
an
a
l
y
s
is
i
n
a
lab
o
r
ato
r
y
s
etti
n
g
a
f
ter
s
a
m
p
lin
g
p
r
o
ce
d
u
r
es.
R
ec
en
t
l
y
,
w
ater
u
tili
tie
s
ad
o
p
ted
f
lu
o
r
esce
n
ce
an
d
ab
s
o
r
b
an
ce
s
p
ec
tr
o
s
co
p
y
tec
h
n
iq
u
es
d
u
e
to
th
eir
ab
ilit
y
to
d
etec
t
b
io
lo
g
ical
q
u
alit
y
m
o
n
i
to
r
in
g
t
h
r
o
u
g
h
o
b
s
er
v
a
tio
n
o
f
f
l
u
o
r
escen
t
d
is
s
o
lv
ed
o
r
g
a
n
ic
m
atter
(
DOM
)
t
h
at
co
r
r
elate
s
to
th
e
p
r
esen
ce
o
f
E
.
co
li
b
ac
ter
ia
[
1
4
]
,
[
1
5
]
.
A
s
tu
d
y
d
e
m
o
n
s
tr
ates
th
e
ab
il
i
t
y
o
f
f
l
u
o
r
escen
ce
s
p
ec
tr
o
s
co
p
y
to
d
etec
t
b
ac
ter
ial
in
tr
in
s
ic
f
lu
o
r
o
p
h
o
r
es
b
ased
o
n
ch
e
m
ical
ce
ll
co
m
p
o
s
i
tio
n
s
:
a
m
i
n
o
ac
id
s
,
f
la
v
in
s
,
a
n
d
n
ico
ti
n
a
m
id
e
n
u
cleo
tid
e
tr
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ase,
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[
1
6
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.
Ho
w
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DOM
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th
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p
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m
p
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ter
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f
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p
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ass
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d
w
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p
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[
1
7
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.
T
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n
tial
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NI
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estru
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w
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th
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p
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t
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tin
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u
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ter
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ex
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[
1
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.
L
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etter
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af
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y
o
f
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w
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ter
s
u
p
p
l
y
.
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h
en
e
v
er
p
o
s
s
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le,
ch
e
m
i
ca
l
s
a
m
p
li
n
g
o
f
t
h
e
w
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s
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ld
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m
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H
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tio
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s
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th
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m
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ial
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m
m
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ities
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t
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e
h
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s
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to
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d
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v
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ce
s
in
r
ea
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ti
m
e
m
o
n
ito
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g
tech
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o
lo
g
y
t
h
at
is
n
ee
d
ed
to
s
tu
d
y
t
h
ese
co
m
m
u
n
ities
e
f
f
ec
ti
v
el
y
.
I
d
ea
ll
y
,
it
is
n
ec
es
s
ar
y
to
estab
lis
h
a
r
eliab
le
m
o
n
i
to
r
in
g
s
tr
ate
g
y
t
h
at
w
o
r
k
s
d
ili
g
en
t
l
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o
r
t
h
e
d
eter
m
i
n
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o
f
t
h
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d
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s
p
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ep
id
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g
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i
n
th
e
ec
o
s
y
s
te
m
.
Ho
w
e
v
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at
s
till
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ep
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ts
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s
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g
n
if
ica
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t
g
ap
in
k
n
o
w
led
g
e.
As
h
i
g
h
lig
h
ted
ab
o
v
e,
av
aila
b
le
w
ater
m
o
n
i
to
r
in
g
tec
h
n
i
q
u
es
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ee
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to
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o
cu
s
o
n
m
o
n
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to
r
in
g
th
e
q
u
alit
y
o
f
w
ater
at
th
e
co
n
s
u
m
p
tio
n
le
v
el.
T
h
e
m
aj
o
r
it
y
o
f
W
QM
ap
p
licatio
n
s
ar
e
p
lace
d
o
n
tr
ea
t
m
e
n
t
f
ac
ilit
ies
an
d
s
e
v
er
al
w
ater
b
ase
s
tatio
n
s
,
co
n
ce
n
tr
ati
n
g
o
n
th
e
q
u
alit
y
o
f
w
ater
r
eso
u
r
ce
s
r
ath
er
th
a
n
th
e
w
ate
r
th
at
i
s
d
eli
v
er
ed
to
u
s
er
s
.
Hen
ce
,
th
is
lead
s
to
d
ata
s
ca
r
cit
y
r
elate
d
to
ev
en
ts
o
f
co
n
ta
m
i
n
at
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n
at
r
esid
e
n
tial
o
r
h
o
u
s
e
h
o
ld
P
OUs.
C
o
n
s
eq
u
e
n
tl
y
,
s
m
all
d
atasets
co
u
ld
n
o
t
p
r
o
v
id
e
a
t
h
o
r
o
u
g
h
i
n
s
ig
h
t
i
n
to
m
icr
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ial
co
n
ta
m
i
n
atio
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e
v
en
ts
w
ith
in
t
h
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W
DS
,
a
s
t
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q
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alit
y
o
f
w
a
ter
co
n
s
ta
n
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y
f
l
u
ctu
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tes
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v
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ti
m
e.
A
d
d
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all
y
,
n
o
q
u
an
ti
tati
v
e
s
t
u
d
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d
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x
p
licit
m
o
n
ito
r
in
g
h
a
v
e
b
ee
n
d
o
n
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in
Ma
la
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icate
d
to
t
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f
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m
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ial
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m
i
n
an
ts
w
it
h
in
t
h
e
h
o
u
s
e
h
o
ld
W
DS
[
1
9
]
.
T
h
e
tr
u
e
d
iv
er
s
it
y
o
f
m
icr
o
b
es
th
at
pr
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ce
in
r
esid
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n
tial
w
a
ter
n
et
w
o
r
k
s
y
s
te
m
s
f
o
r
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n
ce
ca
n
b
e
s
tu
d
ied
,
p
ar
ticu
lar
l
y
at
P
OU
lo
ca
tio
n
s
.
T
h
e
s
cien
t
if
ic
u
n
d
er
s
ta
n
d
in
g
a
n
d
tech
n
o
lo
g
ical
ad
v
a
n
ce
m
e
n
t
n
ec
ess
ar
y
to
ch
ar
ac
ter
ize
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b
es
in
h
o
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s
e
h
o
ld
w
ater
s
y
s
te
m
s
co
m
e
at
a
t
i
m
e
w
h
e
n
t
h
e
n
ee
d
to
m
a
k
e
i
n
f
o
r
m
ed
d
ec
is
io
n
s
ab
o
u
t
u
p
g
r
ad
in
g
a
n
d
o
p
ti
m
iz
in
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q
u
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y
w
ater
s
u
p
p
l
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at
P
OUs
h
as
n
ev
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ee
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.
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to
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ill
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h
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th
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t
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y
o
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f
er
s
an
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s
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l
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th
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e
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f
m
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ial
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m
i
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ater
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tr
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:
−
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ater
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−
No
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g
r
ap
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NI
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R
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m
an
s
p
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t
r
o
s
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p
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ased
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p
r
in
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tr
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s
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Sec
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2.
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Fo
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[
2
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.
W
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alit
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T
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ch
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p
s
p
ec
tr
o
m
e
ter
an
d
t
h
e
HORIB
A
L
ab
R
A
M
H
R
E
v
o
lu
tio
n
m
o
d
e
l.
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h
e
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s
tr
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m
e
n
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al
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t
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n
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8
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m
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ith
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er
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atin
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A
s
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ities
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t
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p
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to
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o
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n
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ater
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ated
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les,
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al
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e
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ith
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ea
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v
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n
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m
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r
o
m
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r
r
esp
o
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2
O
m
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An
y
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esen
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w
t
h
e
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ec
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ied
r
an
g
e,
al
s
o
k
n
o
w
n
as
t
h
e
“f
in
g
e
r
p
r
in
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eg
io
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s
”,
s
p
a
n
n
in
g
f
r
o
m
4
0
0
cm
-
1
to
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8
0
0
cm
-
1
,
i
n
d
icate
p
o
s
s
ib
le
i
m
p
u
r
ities
o
r
o
th
er
s
u
b
s
ta
n
ce
s
in
t
h
e
w
ater
s
a
m
p
le
[
2
5
]
.
Mic
r
o
b
ial
s
p
ec
ies
id
en
ti
f
icatio
n
u
s
in
g
R
a
m
an
s
p
ec
tr
u
m
a
n
al
y
s
is
i
s
b
ased
o
n
s
i
g
n
at
u
r
e
p
ea
k
s
w
it
h
i
n
a
r
an
g
e
o
f
b
an
d
s
as
s
o
ciate
d
w
it
h
ea
c
h
ce
ll
m
ac
r
o
m
o
lec
u
l
e
m
o
n
o
m
er
,
d
ep
ictin
g
d
is
ti
n
c
tiv
e
f
u
n
ctio
n
a
l
g
r
o
u
p
s
[
2
6
]
.
T
h
is
is
d
u
e
to
th
e
v
ib
r
atio
n
al
m
o
d
es
(
s
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m
m
e
tr
i
c,
as
y
m
m
etr
ic,
an
d
b
en
d
i
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g
)
w
it
h
i
n
m
o
lec
u
lar
ce
ll
co
m
p
o
s
itio
n
,
ca
u
s
ed
b
y
r
ad
iatio
n
o
f
lig
h
t
s
in
d
u
ce
d
b
y
R
a
m
a
n
s
p
ec
tr
o
s
co
p
y
,
m
o
d
if
y
in
g
t
h
e
m
o
lec
u
lar
s
tr
u
ct
u
r
e
o
f
s
p
ec
ies
ce
lls
.
Fo
r
in
s
ta
n
ce
,
ch
ar
ac
ter
izi
n
g
s
p
ec
tr
al
s
ig
n
at
u
r
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p
ea
k
s
f
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r
m
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er
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o
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id
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in
m
icr
o
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ial
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s
u
c
h
as
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att
y
ac
id
s
,
ar
e
r
ep
r
esen
ted
in
R
a
m
a
n
s
h
i
f
t
at
1
,
2
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d
1
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c
m
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d
icati
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th
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a
n
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C
=O
s
tr
etc
h
i
n
g
m
o
lecu
lar
p
atter
n
s
[
2
7
]
.
A
n
o
n
-
d
estr
u
cti
v
e
R
a
m
a
n
s
p
ec
tr
o
s
co
p
y
is
a
p
o
w
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f
u
l
m
et
h
o
d
f
o
r
th
e
d
etec
tio
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o
f
m
icr
o
b
ial
co
n
ta
m
i
n
an
t
s
in
wate
r
en
v
ir
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er
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ater
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as e
x
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i
v
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y
d
i
s
cu
s
s
ed
in
[
2
8
]
.
2
.
4
.
Da
t
a
a
na
ly
s
is
T
h
e
Or
ig
in
P
r
o
8
.
6
s
o
f
t
w
ar
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w
a
s
u
s
ed
f
o
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s
tati
s
tical
an
al
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is
a
n
d
i
n
ter
p
r
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o
f
t
h
e
d
is
tr
ib
u
tio
n
p
atter
n
s
f
o
r
q
u
alit
y
m
ea
s
u
r
e
m
en
ts
co
llected
at
r
e
s
id
en
tia
l
P
OUs.
T
h
e
d
ata
p
r
e
-
p
r
o
ce
s
s
in
g
o
f
ac
q
u
ir
ed
R
a
m
a
n
s
p
ec
tr
a
w
a
s
also
ex
ec
u
ted
.
T
h
e
in
itial
s
tep
s
f
o
r
r
ef
i
n
i
n
g
t
h
e
ac
q
u
ir
ed
R
a
m
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s
p
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t
r
u
m
ar
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w
av
e
n
u
m
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lib
r
atio
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,
b
an
d
f
itti
n
g
,
p
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l
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m
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s
m
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ac
k
g
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atab
ase,
Kn
o
w
I
t
A
ll
® in
itiate
s
a
cr
o
s
s
-
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x
a
m
in
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tio
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ch
.
Evaluation Warning : The document was created with Spire.PDF for Python.
T
E
L
KOM
NI
K
A
T
elec
o
m
m
u
n
C
o
m
p
u
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l
C
o
n
tr
o
l
N
o
ve
l in
tern
et
o
f th
in
g
s
-
s
p
ec
tr
o
s
co
p
y
meth
o
d
s
fo
r
ta
r
g
eted
w
a
ter p
o
llu
ta
n
ts
in
…
(
S
ya
h
i
d
a
h
N
u
r
a
n
i Zu
lkifli
)
707
3.
RE
SU
L
T
S AN
D
D
I
SCU
SS
I
O
N
3
.
1
.
A
na
ly
s
is
o
f
pa
ra
m
et
er
s
T
ap
w
ater
s
a
m
p
les
w
er
e
tak
en
i
n
r
esp
o
n
s
e
to
s
ea
s
o
n
al
f
l
u
ctu
a
tio
n
s
,
r
a
n
g
in
g
f
r
o
m
h
ea
v
y
r
ai
n
(
No
v
e
m
b
er
2
0
1
7
to
J
an
u
ar
y
2
0
1
8
)
to
d
r
y
s
u
m
m
er
(
Feb
r
u
ar
y
2
0
1
8
to
A
p
r
il
2
0
1
8
)
w
ea
t
h
e
r
.
Fin
d
in
g
s
s
h
o
w
ed
th
at
al
m
o
s
t
2
.
4
%
o
f
tap
w
a
ter
at
r
esid
en
tial
P
OU
w
a
s
ac
id
ic,
b
elo
w
6
.
5
.
S
ev
en
wate
r
s
a
m
p
les
w
er
e
in
ap
p
r
o
p
r
iate
f
o
r
d
r
in
k
in
g
o
r
d
ail
y
u
s
e,
an
d
t
h
e
s
a
m
p
le
w
a
s
co
llected
b
et
w
ee
n
No
v
e
m
b
er
2
0
1
7
an
d
J
an
u
ar
y
2
0
1
8
.
T
h
e
r
em
ai
n
in
g
w
a
s
w
it
h
in
ac
ce
p
tab
le
r
an
g
e
o
f
p
H
f
r
o
m
p
H
6
.
2
4
to
8
.
5
8
an
d
tem
p
er
atu
r
e
f
r
o
m
2
2
.
0
5
°C
to
3
2
.
1
5
°C
.
T
h
er
e
w
er
e
n
o
i
n
d
icatio
n
s
o
f
tap
w
ater
s
a
m
p
l
es
b
elo
w
6
.
5
b
etw
ee
n
Feb
r
u
a
r
y
2
0
1
8
an
d
A
p
r
il
2
0
1
8
,
s
u
g
g
e
s
ti
n
g
th
at
all
s
a
m
p
les
w
it
h
in
th
a
t
p
er
io
d
ar
e
w
i
th
i
n
ac
ce
p
tab
le
v
al
u
e.
D
u
r
i
n
g
th
e
r
ai
n
y
m
o
n
s
o
o
n
s
ea
s
o
n
(
No
v
e
m
b
er
2
0
1
7
to
J
a
n
u
ar
y
2
0
1
8
)
,
th
e
am
b
ie
n
t
te
m
p
er
atu
r
e
w
as
2
8
°C
,
an
d
th
e
lo
w
est
w
a
s
2
2
°C
.
Me
an
w
h
ile,
t
h
e
p
ea
k
a
n
d
lo
w
est
a
m
b
ie
n
t
te
m
p
er
atu
r
es
th
r
o
u
g
h
o
u
t
th
e
d
r
y
a
n
d
s
u
n
n
y
s
ea
s
o
n
s
(
Feb
r
u
ar
y
2
0
1
8
to
A
p
r
il
2
0
1
8
)
w
er
e
3
3
°C
an
d
2
4
°C
,
r
esp
ec
tiv
el
y
.
Am
b
ie
n
t
te
m
p
er
atu
r
e
w
a
s
m
ea
s
u
r
ed
o
n
th
e
d
a
y
o
f
s
a
m
p
le
co
llectio
n
.
Fro
m
t
h
e
o
b
s
er
v
a
t
io
n
,
th
e
a
v
er
a
g
e
a
m
b
ie
n
t
te
m
p
er
atu
r
e
in
t
h
e
r
ai
n
y
s
ea
s
o
n
(
No
v
e
m
b
er
2
0
1
7
to
J
an
u
ar
y
2
0
1
8
)
w
as
s
ig
n
i
f
ica
n
tl
y
lo
w
er
t
h
an
i
n
t
h
e
w
ar
m
a
n
d
s
u
n
n
y
s
ea
s
o
n
(
Feb
r
u
ar
y
2
0
1
8
to
A
p
r
il
2
0
1
8
)
,
w
h
ic
h
co
u
ld
ex
p
lai
n
th
e
s
lig
h
t
p
H
d
r
o
p
o
b
s
er
v
ed
at
P
O
U
lo
ca
tio
n
s
.
A
n
y
r
ea
d
in
g
s
t
h
at
w
er
e
n
o
t
w
it
h
i
n
ac
ce
p
tab
le
r
an
g
es
f
o
r
tap
w
at
er
,
as
p
r
o
v
id
ed
b
y
en
v
ir
o
n
m
e
n
tal
ag
e
n
cie
s
,
w
er
e
m
ar
k
ed
as
o
u
tlier
s
.
Ou
tlier
s
’
id
en
ti
f
icatio
n
en
ab
les
t
h
e
s
ep
a
r
atio
n
o
f
co
n
ta
m
in
a
ted
w
ater
s
a
m
p
les,
w
h
ic
h
w
er
e
f
u
r
th
er
u
s
ed
f
o
r
co
m
p
o
s
itio
n
an
al
y
s
is
u
s
i
n
g
s
p
ec
tr
o
s
co
p
ic
tech
n
iq
u
es.
T
h
ese
o
u
tlier
s
r
e
p
r
esen
t
ac
tu
al
ev
e
n
t
s
,
s
u
c
h
as
s
p
ik
es
i
n
w
ater
te
m
p
er
atu
r
e
co
r
r
esp
o
n
d
in
g
to
p
H
d
r
o
p
s
ass
o
ciate
d
w
it
h
b
io
lo
g
ical
co
n
ta
m
i
n
atio
n
[
2
9
]
.
A
c
id
ic
w
ater
s
a
m
p
l
e
s
in
t
h
e
d
is
tr
ib
u
t
io
n
s
y
s
te
m
te
n
d
to
h
a
v
e
a
h
i
g
h
er
r
is
k
o
f
m
icr
o
o
r
g
an
i
s
m
co
n
ce
n
tr
atio
n
s
ca
u
s
ed
b
y
f
ae
ca
l
co
n
ta
m
i
n
atio
n
,
s
to
r
m
w
ater
r
u
n
o
f
f
,
w
as
te
e
m
is
s
io
n
s
,
a
n
d
r
a
p
id
u
r
b
an
izatio
n
ac
t
iv
i
ties
.
T
h
er
e
is
co
n
s
id
er
ab
le
ev
id
en
ce
t
h
at
en
v
ir
o
n
m
e
n
tal
f
ac
to
r
s
ca
n
ca
u
s
e
s
u
d
d
en
d
r
o
p
s
in
p
H
lev
els,
r
es
u
lti
n
g
i
n
m
ic
r
o
o
r
g
an
is
m
ac
t
iv
i
t
y
in
d
is
tr
ib
u
tio
n
s
y
s
te
m
s
,
as
s
u
p
p
o
r
ted
b
y
o
th
er
s
tu
d
ies
[
3
0
]
,
[
3
1
]
.
T
ab
le
1
p
r
esen
ts
th
e
ex
tr
e
m
e
p
H
lev
el
an
d
te
m
p
er
atu
r
e
co
n
d
itio
n
s
i
n
r
es
id
en
tial
P
OU
lo
ca
tio
n
s
,
in
cl
u
d
in
g
a
m
b
ie
n
t
te
m
p
er
at
u
r
e
m
ea
s
u
r
ed
d
u
r
in
g
t
h
e
m
o
n
t
h
o
f
s
a
m
p
le
co
llecti
o
n
.
T
ab
le
1
.
E
x
tr
em
e
v
alu
e
o
f
p
H
lev
el
b
elo
w
6
.
5
at
s
ev
er
al
r
esi
d
en
tial P
OU
lo
ca
tio
n
s
S
a
mp
l
i
n
g
mo
n
t
h
s
A
mb
i
e
n
t
t
e
m
p
e
r
a
t
u
r
e
(
H
i
/
L
o
)
,
°C
R
e
si
d
e
n
t
i
a
l
P
O
U
l
o
c
a
t
i
o
n
s
Q
u
a
l
i
t
y
p
a
r
a
me
t
e
r
s
pH
T
e
mp
.
(
°
C
)
N
o
v
e
mb
e
r
2
0
1
7
2
8
/
2
4
L
H
02
6
.
2
7
3
1
.
9
0
L
G
10
6
.
3
2
2
9
.
8
3
D
e
c
e
mb
e
r
2
0
1
7
2
6
/
2
4
L
H
03
6
.
4
8
2
9
.
8
7
L
G
09
6
.
4
6
2
5
.
6
0
Jan
u
a
r
y
2
0
1
8
2
4
/
2
2
L
G
11
6
.
3
5
2
9
.
8
8
L
G
16
6
.
2
4
2
9
.
3
5
L
K09
6
.
3
2
2
8
.
2
1
T
h
e
m
o
s
t
ac
id
ic
w
ater
s
a
m
p
l
es
w
er
e
f
o
u
n
d
in
P
OU
L
G16
,
h
av
i
n
g
a
p
H
d
r
o
p
o
f
6
.
2
4
at
2
9
.
3
5
°C
in
J
an
u
ar
y
2018
w
it
h
th
e
lo
w
est
am
b
ie
n
t
te
m
p
er
at
u
r
e.
T
h
e
h
i
g
h
e
s
t
p
H
w
as
i
n
Dec
e
m
b
er
2
0
1
7
,
w
ith
a
v
al
u
e
o
f
6
.
4
8
an
d
6
.
4
9
in
th
e
P
OU
lo
ca
tio
n
at
L
H03
a
n
d
L
G09
,
r
esp
ec
ti
v
el
y
.
B
ased
o
n
d
is
tr
ib
u
tio
n
al
p
atter
n
s
,
h
i
g
h
w
a
ter
t
e
m
p
er
atu
r
e
co
r
r
esp
o
n
d
ed
to
lo
w
p
H
v
al
u
es
;
a
s
i
m
ilar
tr
en
d
in
o
th
er
f
in
d
i
n
g
s
w
as
al
s
o
p
r
esen
ted
p
r
ev
io
u
s
l
y
[
3
2
]
.
T
h
ese
ex
tr
e
m
e
d
ata
w
er
e
is
o
lated
f
o
r
f
u
r
t
h
er
in
v
est
ig
atio
n
in
to
it
s
co
m
p
o
s
itio
n
to
d
eter
m
i
n
e
w
h
et
h
er
it
co
n
tain
s
an
y
b
io
lo
g
ica
l
p
r
o
p
er
ties
.
L
o
w
p
H
an
d
h
i
g
h
te
m
p
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at
u
r
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i
n
w
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ter
ca
n
in
d
ee
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tr
ig
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ac
ter
ial
p
r
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ctio
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u
t th
e
ex
ten
t o
f
t
h
is
e
f
f
ec
t d
ep
en
d
s
o
n
v
ar
io
u
s
f
ac
to
r
s
.
3.
2
.
Ra
m
a
n s
pect
ru
m
inte
r
p
re
t
a
t
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n
T
h
e
id
en
tif
icatio
n
o
f
ch
ar
ac
ter
is
tic
p
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k
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n
d
icate
s
t
h
at
s
e
v
e
n
w
ater
s
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m
p
le
s
w
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ta
m
i
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ated
w
ith
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ac
ter
ia
s
p
ec
ies
k
n
o
w
n
as
t
h
e
g
r
a
m
-
n
eg
a
tiv
e
Leg
io
n
ella
g
e
n
u
s
.
T
h
e
p
r
ep
r
o
ce
s
s
in
g
o
f
R
a
m
an
s
p
ec
tr
a
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s
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n
g
th
e
f
ir
s
t
d
er
iv
ativ
e
an
d
Sa
v
i
ts
k
y
-
Go
la
y
(
SG)
s
m
o
o
t
h
i
n
g
tech
n
iq
u
e
ar
e
s
h
o
w
n
i
n
Fig
u
r
e
s
1
(
a)
an
d
(
b
)
,
r
esp
ec
tiv
el
y
.
Fi
g
u
r
e
1
(
c)
d
is
p
l
a
y
s
t
h
e
R
a
m
an
s
p
ec
tr
a
f
o
r
tap
w
ater
s
a
m
p
le
s
t
h
at
ar
e
co
n
tam
i
n
ated
at
s
p
ec
i
f
ic
P
OU
lo
ca
tio
n
s
,
n
a
m
el
y
L
H02
, L
G10
,
L
H03
,
L
G11
,
L
G16
,
a
n
d
L
K0
9
.
E
ac
h
lo
ca
tio
n
w
as d
ep
icted
u
s
i
n
g
d
is
t
i
n
ct
co
lo
u
r
co
d
es f
o
r
th
e
ac
q
u
ir
ed
s
p
ec
tr
a,
w
it
h
s
h
ad
ed
r
eg
io
n
s
in
d
icati
n
g
ch
ar
ac
ter
is
tic
p
ea
k
s
f
o
r
Leg
i
o
n
ella
s
p
ec
ies.
T
h
e
R
a
m
a
n
s
p
ec
tr
a
e
x
h
ib
it
d
is
tin
c
t
p
ea
k
s
co
r
r
esp
o
n
d
in
g
to
t
h
e
c
ellu
lar
co
m
p
o
s
itio
n
s
o
f
m
ac
r
o
m
o
lecu
le
s
f
o
u
n
d
in
b
ac
ter
ial
ce
lls
r
ep
r
esen
t
i
n
g
p
o
l
y
s
ac
c
h
ar
id
es,
p
r
o
tein
s
,
a
n
d
n
u
cleic
ac
id
s
[
3
3
]
.
I
n
P
OU
lo
ca
tio
n
s
L
G09
an
d
L
G11
,
h
o
w
ev
er
,
th
er
e
ar
e
s
lig
h
t
s
p
e
ctr
al
in
ten
s
it
y
d
i
f
f
er
en
ce
s
b
etw
ee
n
1
,
2
8
0
cm
-
1
an
d
1
,
3
2
0
c
m
-
1
.
T
h
e
s
ig
n
i
f
ican
t
m
ed
iu
m
-
i
n
te
n
s
it
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ea
k
s
f
all
ar
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u
n
d
b
an
d
1
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3
0
0
cm
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1
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d
g
r
a
d
u
all
y
w
ea
k
er
p
ea
k
s
at
1
,
3
1
7
c
m
-
1
.
Evaluation Warning : The document was created with Spire.PDF for Python.
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23
,
No
.
3
,
J
u
n
e
20
25
:
7
03
-
7
15
708
(
a)
(
b
)
(
c)
Fig
u
r
e
1
.
A
cq
u
ir
ed
R
a
m
a
n
s
p
e
ctr
a
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(
a)
r
aw
s
p
ec
tr
u
m
,
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b
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s
m
o
o
th
ed
SG f
ilter
i
n
g
s
p
ec
tr
u
m
,
an
d
(
c)
Leg
io
n
ella
b
ac
ter
ia
s
p
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ies in
s
e
v
en
P
OU
tap
w
ater
s
a
m
p
le
s
at
lo
ca
tio
n
s
; L
H02
,
L
G10
,
L
H03
,
L
G11
, L
G
16
,
an
d
L
K09
T
h
er
e
ar
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s
u
b
tle
v
ar
iatio
n
s
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th
e
s
p
ec
tr
al
d
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f
f
er
en
ce
s
w
it
h
i
n
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e
r
a
n
g
e
o
f
1
,
6
7
0
cm
-
1
to
1
,
7
0
0
cm
-
1
.
T
h
e
r
eg
io
n
r
ep
r
esen
ts
a
m
id
e
m
o
lecu
lar
v
ib
r
atio
n
s
,
i
n
d
ic
atin
g
a
m
id
e
b
o
n
d
s
’
s
ec
o
n
d
ar
y
s
tr
u
c
tu
r
es
[
3
4
]
.
P
r
ec
is
ely
,
th
e
s
e
ar
ea
s
co
r
r
esp
o
n
d
to
th
e
a
m
id
e
-
t
y
p
e
I
a
n
d
I
I
I
m
o
lecu
lar
b
o
n
d
s
o
f
a
m
i
n
o
a
cid
s
.
T
h
e
h
y
d
r
o
g
e
n
b
o
n
d
in
g
b
et
w
ee
n
a
m
in
o
ac
id
r
esid
u
e
s
tr
u
c
tu
r
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ca
u
s
es
d
if
f
er
en
ce
s
in
ab
s
o
r
b
an
ce
in
te
n
s
it
ie
s
d
u
e
to
d
if
f
er
en
tial
m
o
lecu
lar
p
atter
n
s
a
n
d
g
eo
m
etr
ic
o
r
ien
tatio
n
s
[
3
5
]
.
T
h
e
s
ig
n
a
tu
r
e
p
ea
k
s
id
e
n
ti
f
icatio
n
w
a
s
co
n
d
u
c
ted
b
y
co
m
p
ar
i
n
g
t
h
e
ac
q
u
ir
ed
R
a
m
an
s
h
i
f
t
(
w
a
v
e
n
u
m
b
er
)
to
th
e
w
a
v
en
u
m
b
er
f
r
o
m
t
h
e
R
a
m
a
n
b
an
d
co
r
r
elatio
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tab
le
an
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p
r
ev
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s
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t
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d
ies
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n
d
u
cted
o
n
Leg
io
n
ella
b
ac
ter
ia
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ica
tio
n
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as
s
u
m
m
ar
is
ed
in
T
ab
le
2
.
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h
e
p
r
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m
i
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en
t
R
a
m
an
p
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k
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3
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d
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3
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s
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g
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e
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th
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ce
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H
2
O
b
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d
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r
r
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ater
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o
l
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les
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tr
etc
h
i
n
g
v
ib
r
atio
n
s
[
3
6
]
,
[
3
7
]
.
T
h
e
p
ea
k
s
o
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s
er
v
ed
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b
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d
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7
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9
.
3
7
cm
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d
7
7
6
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5
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1
r
ep
r
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t
p
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ly
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h
ar
id
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m
p
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n
s
i
n
Leg
io
n
ella
s
p
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ies
m
e
m
b
r
a
n
e
ce
ll
s
,
ac
co
m
m
o
d
atin
g
to
s
tr
o
n
g
C
-
H
s
tr
etc
h
i
n
g
v
ib
r
atio
n
s
i
g
n
als
[
3
8
]
.
Ma
j
o
r
p
o
ly
s
ac
c
h
ar
id
e
co
m
p
o
n
e
n
t
s
i
n
m
icr
o
o
r
g
a
n
is
m
ce
ll
m
e
m
b
r
an
es
a
s
s
o
ciate
d
w
i
th
t
h
e
p
ea
k
ce
n
tr
ed
ar
o
u
n
d
7
5
7
cm
-
1
in
d
icate
t
h
e
p
r
esen
ce
o
f
x
y
lo
g
l
u
ca
n
c
h
ain
s
[
3
9
]
.
T
h
e
R
am
a
n
Leg
io
n
ella
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Evaluation Warning : The document was created with Spire.PDF for Python.
I
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:
1
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6930
T
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23
,
No
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3
,
J
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20
25
:
7
03
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7
15
710
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alit
y
.
Mo
r
eo
v
er
,
t
h
e
f
in
d
i
n
g
s
i
n
d
icate
t
h
at
w
ater
q
u
alit
y
alter
atio
n
co
u
ld
p
o
ten
tiall
y
b
e
at
tr
ib
u
ted
to
r
ain
w
ater
r
u
n
-
o
f
f
an
d
g
r
o
u
n
d
w
ater
co
n
ta
m
i
n
atio
n
.
T
h
e
E
P
A
d
ef
in
e
s
b
io
lo
g
ical
q
u
a
liti
es
af
f
ec
ted
b
y
te
m
p
er
atu
r
e
f
l
u
ct
u
atio
n
s
i
n
wate
r
b
o
d
ies
as
o
r
g
an
is
m
s
u
r
v
i
v
al,
g
r
o
w
t
h
,
r
ep
r
o
d
u
ctio
n
,
d
ev
elo
p
m
e
n
t,
b
e
h
av
io
r
,
an
d
h
ab
itat
p
r
ef
er
en
ce
s
[
4
7
]
.
Du
r
i
n
g
in
c
le
m
e
n
t
w
ea
th
er
co
n
d
itio
n
s
s
u
ch
a
s
s
to
r
m
s
o
r
r
ain
f
al
l
p
er
io
d
s
,
r
ain
w
ater
tr
a
v
er
s
es
o
v
er
e
x
p
o
s
ed
ter
r
estrial
an
d
aq
u
atic
s
u
r
f
ac
es.
Ma
n
y
p
r
ev
io
u
s
s
t
u
d
ies
h
a
v
e
s
h
o
w
n
s
ig
n
i
f
ica
n
t
w
ater
ec
o
lo
g
y
i
m
p
ac
ts
d
er
iv
ed
f
r
o
m
r
ai
n
w
ater
c
o
m
p
o
n
en
t
s
.
T
h
ese
i
m
p
ac
t
s
in
clu
d
e
th
e
d
is
co
v
er
y
o
f
in
o
r
g
an
ic
io
n
s
p
ec
ies
d
u
r
in
g
m
o
n
s
o
o
n
p
er
io
d
s
[
4
8
]
,
ch
e
m
ica
l
co
m
p
o
s
itio
n
s
[
4
9
]
,
an
d
in
d
u
s
tr
ial
co
m
b
u
s
t
io
ns
[
5
0
]
.
A
cc
o
r
d
in
g
to
th
e
p
H
lev
els
f
o
u
n
d
in
No
v
e
m
b
er
2
0
1
7
,
Dec
em
b
er
2
0
1
7
,
an
d
J
an
u
ar
y
2
0
1
8
,
s
ev
e
n
w
a
ter
s
a
m
p
les
w
er
e
n
o
t
w
ith
in
an
ac
ce
p
tab
le
r
an
g
e.
A
n
y
r
ea
d
i
n
g
s
th
at
w
er
e
n
o
t
w
ith
in
ac
ce
p
tab
le
r
an
g
e
s
f
o
r
tap
w
ater
,
as p
r
o
v
id
ed
b
y
e
n
v
ir
o
n
m
e
n
tal
a
g
e
n
cies,
w
er
e
m
ar
k
ed
as o
u
tlier
s
.
T
h
ese
o
u
tl
i
e
r
s
r
e
p
r
e
s
e
n
t
a
c
t
u
a
l
ev
en
ts
,
s
u
ch
as
s
p
ik
es
i
n
w
at
e
r
t
em
p
e
r
at
u
r
e
c
o
r
r
e
s
p
o
n
d
i
n
g
t
o
p
H
d
r
o
p
s
a
s
s
o
c
i
at
e
d
w
i
th
b
i
o
l
o
g
i
ca
l
c
o
n
t
am
in
a
t
i
o
n
.
T
h
e
r
e
i
s
c
o
n
s
i
d
e
r
a
b
l
e
ev
i
d
en
c
e
th
a
t
e
n
v
i
r
o
n
m
e
n
t
al
f
a
ct
o
r
s
c
an
c
au
s
e
s
u
d
d
en
d
r
o
p
s
in
p
H
l
ev
e
ls
,
r
e
s
u
l
t
in
g
i
n
m
ic
r
o
o
r
g
an
i
s
m
ac
t
i
v
i
ty
in
d
is
t
r
i
b
u
t
i
o
n
s
y
s
t
em
s
.
D
e
p
e
n
d
i
n
g
o
n
th
e
ty
p
e
o
f
b
a
c
t
e
r
i
a
s
p
e
c
ie
s
an
d
a
c
i
d
i
ty
t
o
le
r
a
n
c
e
,
l
o
w
p
H
l
ev
e
l
s
c
an
p
r
o
m
o
t
e
b
a
ct
e
r
i
a
l
p
r
o
d
u
c
t
i
o
n
an
d
g
r
o
w
th
.
S
ev
e
r
a
l
b
a
c
t
e
r
i
a
s
p
e
c
i
es
c
an
w
ith
s
t
an
d
a
ci
d
i
c
w
a
t
e
r
c
o
n
d
i
tio
n
s
,
s
u
ch
a
s
S
tr
e
p
t
o
c
o
c
c
u
s
g
en
u
s
,
A
c
i
d
o
b
a
c
t
er
i
u
m
c
a
p
s
u
l
a
t
u
m
,
E
s
c
h
e
r
i
c
h
i
a
c
o
l
i
,
a
n
d
P
r
o
p
i
o
n
i
b
a
c
t
er
i
u
m
a
c
i
d
i
p
r
o
p
i
o
n
i
c
i
[
5
1
]
.
W
a
t
e
r
d
i
s
t
r
i
b
u
ti
o
n
p
i
p
e
l
in
e
n
e
tw
o
r
k
s
p
r
o
v
i
d
e
a
n
i
d
e
a
l
e
n
v
i
r
o
n
m
en
t
f
o
r
b
a
c
t
e
r
ia
t
o
r
e
p
r
o
d
u
c
e
an
d
a
f
e
r
ti
l
e
en
v
i
r
o
n
m
en
t
f
o
r
b
ac
t
e
r
iu
m
g
r
o
w
th
.
T
h
i
s
i
s
d
u
e
t
o
s
ev
e
r
a
l
g
r
o
w
th
-
p
r
o
m
o
t
in
g
n
u
t
r
i
en
ts
th
at
a
l
l
o
w
b
a
c
t
e
r
i
a
c
o
m
m
u
n
i
ti
e
s
t
o
s
u
r
v
iv
e
,
e
v
en
in
l
o
w
-
o
x
y
g
en
en
v
i
r
o
n
m
en
ts
.
3.
3
.
P
r
o
po
s
ed
m
icro
-
s
pect
ro
m
et
er
co
nfig
ura
t
io
n
E
ac
h
p
h
ase
o
f
th
e
h
ar
d
w
ar
e
d
esig
n
p
r
o
ce
s
s
co
n
s
is
ts
o
f
th
r
ee
p
r
im
ar
y
co
m
p
o
n
en
t
s
:
th
e
R
a
m
an
o
p
tical
s
etu
p
,
t
h
e
s
p
ec
tr
o
m
eter
s
y
s
te
m
,
an
d
t
h
e
w
ater
q
u
a
lit
y
m
o
n
i
to
r
in
g
w
eb
s
er
v
er
.
T
h
ese
co
m
p
o
n
en
ts
ar
e
d
ep
icted
in
Fi
g
u
r
e
4
.
E
ac
h
ele
m
en
t
co
n
s
i
s
ts
o
f
cr
u
cia
l
cr
iter
ia
u
s
ed
in
th
e
d
ev
elo
p
m
e
n
t
o
f
a
co
m
p
r
eh
en
s
i
v
e
p
o
r
tab
le
s
p
ec
tr
o
m
e
ter
in
te
g
r
ated
w
it
h
a
clo
u
d
-
b
ased
w
ater
q
u
a
lit
y
m
o
n
ito
r
i
n
g
s
y
s
te
m
.
Se
v
er
al
d
esig
n
p
ar
a
m
eter
s
t
h
at
w
er
e
co
n
s
id
er
ed
ar
e
R
am
a
n
o
p
tical
co
n
f
ig
u
r
atio
n
s
,
m
ater
i
als
s
elec
tio
n
an
d
len
s
d
esig
n
,
d
if
f
r
ac
tio
n
g
r
ati
n
g
an
d
lig
h
t
d
etec
to
r
s
p
ec
if
icatio
n
.
T
h
ese
p
a
r
am
eter
s
af
f
ec
t
th
e
o
u
tco
m
e
o
f
R
a
m
a
n
s
p
ec
tr
al
r
eso
lu
tio
n
an
d
s
p
ec
tr
o
m
e
ter
s
en
s
iti
v
it
y
;
t
h
er
ef
o
r
e,
en
s
u
r
in
g
o
p
ti
m
al
d
esi
g
n
p
ar
am
eter
s
i
s
ess
e
n
tia
l.
As
f
o
r
th
e
d
ev
elo
p
m
en
t
o
f
a
w
eb
-
b
ased
q
u
alit
y
m
o
n
ito
r
in
g
s
er
v
er
,
it
is
i
m
p
er
ati
v
e
to
en
s
u
r
e
o
v
er
all
s
y
s
te
m
p
er
f
o
r
m
a
n
ce
s
ar
e
s
atis
f
ac
to
r
y
,
p
ar
ticu
lar
l
y
i
n
d
ata
ac
q
u
is
itio
n
ti
m
e
an
d
r
ea
l
-
ti
m
e
r
e
m
o
te
s
en
s
i
n
g
ca
p
ab
ilit
ies.
T
h
e
s
u
g
g
e
s
ted
d
ev
ice
is
s
m
all,
li
g
h
t
w
eig
h
t,
an
d
co
m
p
ac
t,
o
f
f
er
i
n
g
co
s
t
-
e
f
f
ec
ti
v
e
o
n
-
s
ite
m
ea
s
u
r
e
m
en
ts
th
at
en
ab
le
r
e
m
o
te
d
etec
tio
n
o
f
m
icr
o
o
r
g
an
is
m
c
o
n
ta
m
i
n
a
n
ts
in
r
esid
en
t
ial
o
r
h
o
u
s
e
h
o
ld
p
ip
elin
e
s
y
s
te
m
s
,
i
n
clu
d
i
n
g
tap
w
ater
.
W
ith
its
w
ir
eless
ca
p
ab
ilit
y
a
n
d
r
ea
l
-
ti
m
e
d
ata
tr
a
n
s
f
er
u
s
in
g
i
n
ter
n
et
o
f
th
in
g
s
(
I
o
T
)
in
teg
r
atio
n
tec
h
n
o
lo
g
y
,
th
is
m
i
n
iat
u
r
e
s
p
ec
tr
o
m
eter
s
i
m
p
li
f
ies
w
ater
q
u
al
it
y
m
ea
s
u
r
e
m
en
ts
,
m
ak
i
n
g
th
e
m
q
u
ick
a
n
d
s
tr
aig
h
t
f
o
r
w
ar
d
.
T
h
e
h
ar
d
w
ar
e
is
d
esig
n
ed
to
alig
n
w
i
th
f
a
u
ce
t
m
ea
s
u
r
e
m
e
n
ts
f
o
r
tap
w
ater
.
C
o
n
s
id
er
ati
o
n
o
f
p
ip
e
w
id
t
h
s
an
d
w
a
ter
p
r
o
o
f
in
g
f
e
atu
r
es
i
s
cr
u
cial
d
u
r
in
g
w
at
er
s
a
m
p
le
a
n
al
y
s
is
.
T
h
e
No
d
eM
C
U
f
ir
m
w
ar
e
f
ac
ilit
ate
s
d
ata
ac
q
u
is
itio
n
t
o
an
d
f
r
o
m
Am
az
o
n
clo
u
d
n
et
w
o
r
k
s
,
w
h
ich
ar
e
u
til
ized
f
o
r
I
o
T
d
atab
ase
m
an
a
g
e
m
e
n
t
s
y
s
te
m
s
.
T
h
is
p
r
o
p
o
s
ed
h
ar
d
w
ar
e
o
f
f
er
s
u
n
iq
u
e
f
ea
t
u
r
es
:
i)
f
ac
i
litati
n
g
r
ea
l
-
ti
m
e
d
etec
ti
o
n
o
f
b
ac
ter
ial
co
n
ta
m
in
at
io
n
ev
e
n
ts
in
h
o
u
s
eh
o
ld
w
ater
s
u
p
p
ly
p
ip
elin
e
s
y
s
te
m
s
,
ii)
en
h
a
n
cin
g
w
ater
q
u
alit
y
m
an
a
g
e
m
e
n
t
f
o
r
d
ec
is
io
n
-
m
a
k
er
s
,
iii)
e
n
s
u
r
in
g
s
a
f
et
y
f
o
r
co
n
s
u
m
er
u
s
a
g
e,
an
d
i
v
)
en
a
b
lin
g
b
o
th
e
n
d
-
u
s
er
s
an
d
w
a
ter
s
ta
k
eh
o
ld
er
s
to
s
y
n
c
h
r
o
n
ize
co
n
t
a
m
in
at
io
n
e
v
en
t
o
cc
u
r
r
en
ce
s
.
A
d
d
itio
n
a
l
l
y
,
it
is
co
m
p
ac
t,
lig
h
t
w
ei
g
h
t,
an
d
ea
s
y
to
u
s
e.
T
h
is
s
y
s
te
m
i
n
cl
u
d
es
a
d
etac
h
ab
le
cu
s
to
m
ized
h
o
llo
w
f
au
ce
t
co
n
n
ec
tio
n
t
h
at
ca
n
b
e
in
s
talled
o
n
v
ar
io
u
s
p
ip
es,
s
u
ch
as
h
ea
d
s
h
o
w
er
s
,
to
ilet
f
au
ce
ts
,
o
r
w
a
s
h
in
g
m
ac
h
i
n
e
w
ater
s
u
p
p
l
y
.
On
ce
w
ater
f
lo
w
s
t
h
r
o
u
g
h
t
h
is
h
o
ll
o
w
co
n
n
ec
to
r
,
a
m
e
s
h
s
ep
ar
at
o
r
f
u
n
ctio
n
s
to
co
llect
a
w
ate
r
s
am
p
le
f
o
r
test
i
n
g
p
u
r
p
o
s
es.
T
o
en
s
u
r
e
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ilar
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Evaluation Warning : The document was created with Spire.PDF for Python.