I
nte
rna
t
io
na
l J
o
urna
l o
f
Adv
a
nces in Applie
d Science
s
(
I
J
AAS)
Vo
l.
1
3
,
No
.
4
,
Dec
em
b
er
2
0
2
4
,
p
p
.
8
1
8
~
82
4
I
SS
N:
2
2
5
2
-
8
8
1
4
,
DOI
: 1
0
.
1
1
5
9
1
/ijaas.v
1
3
.
i
4
.
pp
818
-
82
4
818
J
o
ur
na
l ho
m
ep
a
g
e
:
h
ttp
:
//ij
a
a
s
.
ia
esco
r
e.
co
m
Cha
ra
cter
isti
c of
bla
ck
so
y
miso
wit
h cru
de brom
ela
i
n and
Lacto
b
a
cillus p
la
nta
rum
B
ha
k
t
i Etz
a
Set
ia
ni,
Yo
y
o
k
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ud
i P
ra
m
o
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nto
ro
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a
m
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ng
Dwilo
k
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Anni
s
a
Sh
a
f
a
P
utr
i A
rini,
I
lm
a
M
uli
a
s
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ri
Ra
m
a
dh
a
nin
g
rum
,
E
lis
a
bet
h F
ebria
ne
L
o
v
it
a
Su
djo
no
F
o
o
d
Te
c
h
n
o
l
o
g
y
P
r
o
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m
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p
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A
g
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d
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g
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c
u
l
t
u
r
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c
i
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n
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e
s,
D
i
p
o
n
e
g
o
r
o
U
n
i
v
e
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si
t
y
,
S
e
mara
n
g
,
I
n
d
o
n
e
si
a
Art
icle
I
nfo
AB
S
T
RAC
T
A
r
ticle
his
to
r
y:
R
ec
eiv
ed
J
an
2
7
,
2
0
2
4
R
ev
is
ed
J
u
n
6
,
2
0
2
4
Acc
ep
ted
J
u
n
1
9
,
2
0
2
4
Th
is
re
se
a
rc
h
a
ims
to
imp
r
o
v
e
th
e
d
i
v
e
rsity
o
f
f
u
n
c
ti
o
n
a
l
fo
o
d
s
rich
i
n
b
io
a
c
ti
v
e
p
e
p
t
id
e
c
o
m
p
o
n
e
n
ts
a
n
d
h
a
v
e
a
u
n
iq
u
e
flav
o
r
f
o
r
b
lac
k
so
y
b
e
a
n
m
iso
th
ro
u
g
h
t
h
e
a
d
d
it
i
o
n
o
f
c
r
u
d
e
b
r
o
m
e
lain
a
n
d
L
a
c
to
b
a
c
il
l
u
s
p
la
n
ta
r
u
m
.
Th
is
re
se
a
rc
h
u
se
s
a
c
o
m
p
lete
ly
r
a
n
d
o
m
ize
d
d
e
sig
n
wit
h
5
trea
tme
n
ts
a
n
d
4
re
p
e
ti
ti
o
n
s
t
o
o
b
tai
n
a
to
tal
o
f
2
0
e
x
p
e
rime
n
tal
u
n
it
s.
Th
e
trea
tme
n
ts
a
re
t
h
e
d
iffere
n
t
c
o
n
c
e
n
trati
o
n
s
o
f
c
ru
d
e
b
ro
m
e
lain
a
d
d
it
i
o
n
,
n
a
m
e
ly
t
h
e
c
o
n
tro
l
trea
tme
n
t
o
r
0
%
(T
0
),
3
%
(T
1
),
6
%
(T
2
),
9
%
(T
3
),
a
n
d
1
2
%
(T4
).
T
h
e
p
a
ra
m
e
ter
s
o
b
se
rv
e
d
a
re
wa
ter
c
o
n
ten
t,
wa
ter
a
c
ti
v
it
y
,
p
H
v
a
l
u
e
,
a
n
d
lac
ti
c
a
c
id
b
a
c
teria
(LAB)
v
iab
il
it
y
.
T
h
e
re
su
lt
sh
o
we
d
t
h
a
t
a
n
i
n
c
re
a
se
in
th
e
c
ru
d
e
b
ro
m
e
lain
c
o
n
c
e
n
tratio
n
m
a
y
i
n
c
r
e
a
se
th
e
wa
ter co
n
ten
t
a
n
d
wa
ter ac
ti
v
it
y
o
f
th
e
b
lac
k
so
y
b
e
a
n
m
iso
,
wh
il
e
th
e
p
H v
a
lu
e
d
e
c
re
a
se
s
.
Ba
se
d
o
n
th
e
v
iab
il
it
y
o
f
th
e
LAB
sh
o
we
d
a
wa
v
e
rin
g
re
su
lt
.
M
iso
wi
th
t
h
e
9
%
c
o
n
c
e
n
tratio
n
o
f
c
ru
d
e
b
r
o
m
e
lain
is t
h
e
b
e
st r
e
su
lt
a
m
o
n
g
t
h
e
o
t
h
e
rs.
K
ey
w
o
r
d
s
:
B
lack
s
o
y
b
ea
n
C
r
u
d
e
b
r
o
m
elain
Fer
m
en
tatio
n
Miso
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
T
h
is i
s
a
n
o
p
e
n
a
c
c
e
ss
a
rticle
u
n
d
e
r th
e
CC B
Y
-
SA
li
c
e
n
se
.
C
o
r
r
e
s
p
o
nd
ing
A
uth
o
r
:
B
h
ak
ti E
tza
Setian
i
Fo
o
d
T
ec
h
n
o
lo
g
y
Pro
g
r
am
,
D
ep
ar
tm
en
t o
f
Ag
r
icu
ltu
r
al
Scien
ce
Facu
lty
o
f
An
im
al
an
d
Ag
r
ic
u
ltu
r
al
Scien
ce
s
,
Dip
o
n
eg
o
r
o
U
n
iv
er
s
ity
Pro
f
So
ed
ar
to
SH
St.
T
em
b
alan
g
,
Sem
ar
an
g
,
5
0
2
7
5
,
I
n
d
o
n
esia
E
m
ail: b
h
ak
tietzasetian
i@
lect
u
r
er
.
u
n
d
ip
.
ac
.
id
1.
I
NT
RO
D
UCT
I
O
N
Miso
is
a
tr
ad
itio
n
al
J
ap
an
ese
co
n
d
im
en
t in
th
e
f
o
r
m
o
f
s
o
y
b
ea
n
p
aste w
h
ich
co
m
es f
r
o
m
t
h
e
p
r
o
ce
s
s
o
f
m
ix
i
n
g
b
o
iled
s
o
y
b
ea
n
s
,
s
alt
,
an
d
k
o
ji
cu
ltu
r
e
wh
ich
is
f
er
m
en
ted
in
th
r
ee
to
twen
ty
-
f
o
u
r
m
o
n
th
s
[
1
]
.
Miso
is
o
n
e
o
f
t
h
e
s
o
y
b
ea
n
s
p
aste
th
at
is
p
r
o
d
u
ce
d
b
y
f
e
r
m
en
tin
g
s
o
y
b
ea
n
s
with
b
r
in
e
a
n
d
k
o
ji
m
o
ld
u
n
d
er
s
p
ec
if
ic
h
u
m
id
ity
a
n
d
tem
p
er
atu
r
es
[
2
]
.
T
h
e
p
r
o
ce
s
s
o
f
m
a
k
in
g
m
is
o
tak
es
m
o
n
th
s
b
ec
au
s
e
it
g
o
es
th
r
o
u
g
h
a
f
er
m
en
tatio
n
p
r
o
ce
s
s
to
o
b
tain
o
p
tim
al
n
u
tr
itio
n
al
v
alu
e,
a
m
o
u
n
t
o
f
lactic
ac
id
b
ac
ter
ia
(
L
AB
)
,
f
lav
o
r
,
an
d
o
th
er
s
u
p
p
o
r
tin
g
f
ac
to
r
s
[
3
]
.
Miso
h
as
m
an
y
b
en
e
f
its
f
o
r
t
h
e
h
u
m
a
n
b
o
d
y
b
ec
au
s
e
it
co
n
tain
s
is
o
f
lav
o
n
es
wh
ich
ar
e
an
tio
x
id
an
t
co
m
p
o
u
n
d
s
f
o
u
n
d
in
s
o
y
b
ea
n
s
[
4
]
.
Ap
ar
t
f
r
o
m
b
ein
g
r
ich
in
an
tio
x
id
an
ts
,
m
is
o
also
h
a
s
m
an
y
n
u
tr
itio
n
al
co
n
ten
ts
.
T
h
e
n
u
tr
itio
n
al
co
n
t
en
t
o
f
m
i
s
o
ca
n
b
e
in
cr
ea
s
ed
f
u
r
th
er
b
y
ch
an
g
i
n
g
th
e
b
asic
in
g
r
ed
ien
t,
n
am
el
y
y
ello
w
s
o
y
b
ea
n
s
t
o
b
lack
s
o
y
b
ea
n
s
.
B
lack
s
o
y
b
ea
n
s
h
av
e
th
eir
o
wn
ad
v
a
n
tag
es
b
ec
au
s
e
th
ey
h
a
v
e
q
u
ite
h
ig
h
n
u
tr
itio
n
al
co
n
ten
t,
esp
ec
ially
p
r
o
tein
a
n
d
ca
r
b
o
h
y
d
r
ates
[
5
]
.
T
h
e
g
l
u
tam
ic
ac
id
co
n
ten
t
i
n
b
lack
s
o
y
b
ea
n
s
is
also
h
ig
h
er
wh
en
co
m
p
ar
e
d
to
y
ello
w
s
o
y
b
ea
n
s
.
T
h
ese
am
in
o
ac
id
s
ar
e
co
m
p
o
n
en
ts
th
at
f
o
r
m
th
e
s
av
o
r
y
tast
e
o
f
m
is
o
[
6
]
.
T
h
e
p
r
o
ce
s
s
o
f
m
ak
in
g
m
is
o
will
in
v
o
lv
e
a
f
er
m
en
tatio
n
p
r
o
ce
s
s
,
wh
er
e
th
e
p
r
o
ce
s
s
ca
n
b
e
ac
ce
ler
ated
b
y
a
d
d
in
g
cr
u
d
e
b
r
o
m
elain
en
z
y
m
e
b
ec
a
u
s
e
m
icr
o
b
es
d
o
n
o
t
n
ee
d
to
p
r
o
d
u
c
e
p
r
o
tease
en
zy
m
es
to
b
r
ea
k
d
o
wn
p
r
o
tein
s
.
Evaluation Warning : The document was created with Spire.PDF for Python.
I
n
t J Ad
v
Ap
p
l Sci
I
SS
N:
2252
-
8
8
1
4
C
h
a
r
a
cteris
tic
o
f b
la
ck
s
o
y
mi
s
o
w
ith
cru
d
e
b
r
o
mela
in
a
n
d
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
…
(
B
h
a
kti
E
tz
a
S
etia
n
i
)
819
T
h
e
p
r
o
tease
en
zy
m
e
co
n
tain
ed
in
t
h
e
b
r
o
m
elain
en
zy
m
e
i
s
f
o
u
n
d
in
p
in
ea
p
p
le
an
d
ca
n
h
y
d
r
o
ly
ze
p
ep
tid
e
b
o
n
d
s
in
p
r
o
tein
s
in
to
s
im
p
ler
m
o
lecu
les,
n
a
m
ely
a
m
in
o
ac
id
s
,
m
a
k
in
g
th
em
ea
s
ier
f
o
r
th
e
b
o
d
y
to
d
ig
est
[
7
]
.
Pro
tease
en
zy
m
es
ca
n
also
b
e
f
o
u
n
d
in
p
ap
ay
a
f
r
u
it,
n
a
m
ely
th
e
p
ap
ai
n
en
z
y
m
e,
b
u
t
th
e
p
ap
ain
en
zy
m
e
will
g
iv
e
a
b
itter
tast
e
s
o
it
is
n
o
t
s
u
itab
le
if
ad
d
ed
to
m
is
o
p
r
o
d
u
cts
[
8
]
.
An
o
th
er
ad
v
an
tag
e
o
f
u
s
in
g
th
e
b
r
o
m
elai
n
en
zy
m
e
is
th
at
it
ca
n
s
p
ee
d
u
p
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
.
T
h
e
m
is
o
f
er
m
e
n
tatio
n
p
r
o
ce
s
s
ca
n
b
e
f
u
r
th
er
ac
ce
le
r
ated
b
y
ad
d
i
n
g
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
[
9
]
.
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
is
o
n
e
o
f
th
e
L
AB
s
p
ec
ies
th
at
is
o
f
ten
u
s
ed
f
o
r
f
er
m
e
n
tatio
n
b
ec
au
s
e
o
f
its
am
y
lo
ly
tic
p
r
o
p
er
ties
[
1
0
]
.
T
h
e
p
r
esen
ce
o
f
am
y
lo
ly
tic
ac
t
iv
ity
o
f
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
will
b
e
ab
le
to
p
r
o
d
u
ce
m
o
r
e
g
lu
c
o
s
e,
th
en
th
e
g
lu
c
o
s
e
will
b
e
co
n
v
er
ted
in
to
p
y
r
u
v
ate
b
y
f
r
ee
in
g
wate
r
m
o
lecu
les
[
1
1
]
.
T
h
e
m
icr
o
b
io
lo
g
ical
am
y
lo
ly
tic
a
ctiv
ity
o
f
L
AB
wh
ich
ca
n
h
y
d
r
o
ly
ze
s
tar
ch
in
to
lactic
ac
id
b
y
f
r
ee
in
g
wate
r
m
o
lecu
les
ca
n
ca
u
s
e
th
e
wat
er
co
n
ten
t
to
i
n
cr
ea
s
e
[
1
2
]
.
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
h
as
a
h
ig
h
er
in
h
ib
ito
r
y
p
o
wer
co
m
p
ar
e
d
to
o
th
er
ty
p
es
o
f
L
AB
in
in
h
ib
itin
g
t
h
e
g
r
o
wth
o
f
p
at
h
o
g
e
n
ic
b
a
cter
ia.
L
ac
tic
ac
id
m
etab
o
lite
co
m
p
o
u
n
d
s
will
b
e
p
r
o
d
u
ce
d
f
r
o
m
o
p
tim
al
L
AB
g
r
o
wth
wh
ich
ca
n
lo
wer
th
e
p
H
t
h
er
eb
y
in
cr
ea
s
in
g
ac
id
ity
b
ec
a
u
s
e
lactic
ac
id
d
is
s
o
ciate
s
in
th
e
f
o
r
m
o
f
H
+
[
1
3
]
.
I
n
th
e
p
r
o
ce
s
s
o
f
m
a
k
in
g
m
is
o
,
s
alt
is
ad
d
ed
t
o
k
ee
p
an
o
th
e
r
m
icr
o
b
e
b
esid
es
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
f
r
o
m
g
r
o
w
in
g
[
1
4
]
.
Ap
ar
t
f
r
o
m
th
at,
in
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
s
alt
also
p
lay
s
a
r
o
le
in
b
in
d
in
g
wate
r
an
d
f
o
r
m
in
g
th
e
tast
e
o
f
m
is
o
.
T
h
e
wate
r
i
n
th
e
p
r
o
d
u
ct
will
b
e
b
o
u
n
d
b
y
s
alt
s
o
t
h
at
it
h
as
v
alu
e
a
w
d
ec
r
ea
s
ed
b
ec
a
u
s
e
s
alt
is
h
y
g
r
o
s
co
p
ic
[
1
5
]
.
I
n
g
en
e
r
al,
o
p
tim
al
en
v
ir
o
n
m
en
tal
co
n
d
itio
n
s
f
o
r
b
ac
ter
ia
to
g
r
o
w
an
d
d
ev
elo
p
h
a
v
e
a
v
alu
e
a
w
r
an
g
es
f
r
o
m
0
.
6
to
0
.
9
.
T
h
e
ex
i
s
ten
ce
o
f
t
h
is
attac
h
m
en
t
m
ak
es
r
esear
ch
er
s
wan
t
to
k
n
o
w
t
h
e
ef
f
ec
t
o
f
th
e
a
d
d
it
io
n
o
f
c
r
u
d
e
b
r
o
m
elain
en
z
y
m
e
o
n
wate
r
co
n
ten
t,
wate
r
ac
ti
v
ity
,
p
H
v
al
u
e,
an
d
L
AB
v
iab
ilit
y
.
2.
RE
S
E
ARCH
M
E
T
H
O
D
2
.
1
.
Resea
rc
h desi
g
n
Sam
p
les
wer
e
p
r
e
p
ar
ed
in
a
co
m
p
letely
r
an
d
o
m
ize
d
d
es
ig
n
wh
ich
was
ca
r
r
ie
d
o
u
t
in
s
tag
es
in
clu
d
in
g
:
i
)
p
r
e
p
ar
atio
n
o
f
cr
u
d
e
b
r
o
m
elain
,
ii
)
m
an
u
f
ac
tu
r
in
g
o
f
b
lac
k
s
o
y
m
is
o
,
an
d
iii
)
co
m
p
a
r
ativ
e
an
aly
s
is
o
f
ch
em
ical
an
d
m
icr
o
b
ial
p
ar
am
eter
s
o
f
b
lac
k
s
o
y
m
is
o
.
T
h
e
tr
ea
tm
en
t
g
iv
en
to
b
lack
s
o
y
m
is
o
is
th
e
cr
u
d
e
b
r
o
m
elain
co
n
ce
n
tr
atio
n
o
f
0
%
as
co
n
tr
o
l,
3
,
6
,
9
,
an
d
1
2
%.
T
h
is
r
esear
ch
was
ca
r
r
ied
o
u
t
at
th
e
Fo
o
d
C
h
em
is
tr
y
an
d
Nu
tr
itio
n
L
ab
o
r
ato
r
y
an
d
t
h
e
Fo
o
d
E
n
g
in
ee
r
in
g
an
d
Ag
r
icu
ltu
r
al
P
r
o
d
u
cts,
Facu
lty
o
f
An
im
al
an
d
Ag
r
ic
u
ltu
r
e,
Un
i
v
er
s
itas
Dip
o
n
eg
o
r
o
f
r
o
m
J
u
n
e
to
Sep
tem
b
er
2
0
2
3
.
T
h
e
m
ain
in
g
r
ed
ien
ts
in
t
h
is
s
tu
d
y
ar
e
b
lack
s
o
y
b
ea
n
s
(
5
0
0
g
)
o
r
d
er
ed
f
r
o
m
Gan
g
B
ar
u
Ma
r
k
et,
Kr
an
g
g
an
,
C
en
tr
al
Se
m
ar
an
g
,
Sem
a
r
an
g
,
with
th
e
v
ar
iety
u
s
ed
b
ein
g
M
allik
a
an
d
p
in
ea
p
p
les
o
b
tain
e
d
f
r
o
m
Dam
ar
Ma
r
k
et,
Pad
an
g
s
ar
i,
B
an
y
u
m
an
ik
,
Sem
ar
an
g
.
2
.
2
.
Resea
rc
h pro
ce
du
re
2
.
2
.
1
.
P
re
pa
ra
t
i
o
n o
f
cr
ud
e
bro
m
ela
in
T
h
e
p
r
ep
ar
atio
n
m
et
h
o
d
o
f
cr
u
d
e
b
r
o
m
elain
h
as
b
ee
n
ad
o
p
t
ed
f
r
o
m
[
1
6
]
,
with
m
o
d
if
icati
o
n
s
.
First,
p
in
ea
p
p
les
wer
e
p
ee
le
d
o
f
f
a
n
d
s
ep
ar
ated
f
r
o
m
th
e
b
r
a
cts
th
en
cu
t
in
t
o
s
m
all
p
iece
s
an
d
m
ash
ed
u
s
in
g
a
b
len
d
er
.
T
h
e
b
len
d
e
r
s
p
ee
d
m
u
s
t
b
e
at
th
e
lo
west
o
r
o
n
a
s
ca
le
o
f
1
,
s
o
th
e
p
in
ea
p
p
l
es
m
ash
ed
ca
r
ef
u
lly
.
0
.
1
M
p
h
o
s
p
h
ate
b
u
f
f
er
p
H
7
was
ad
d
ed
to
m
ash
ed
p
i
n
ea
p
p
les
in
a
1
:1
r
atio
g
r
a
d
u
ally
.
T
h
e
p
in
ea
p
p
le
ju
ice
was
f
ilter
ed
u
s
in
g
a
f
ilter
clo
t
h
an
d
f
o
llo
we
d
b
y
ce
n
tr
if
u
g
at
io
n
at
4
5
0
0
r
p
m
f
o
r
1
5
m
i
n
u
t
es
to
s
ep
ar
ate
th
e
s
u
p
er
n
atan
t a
n
d
th
e
p
ellet
s
o
th
at
g
o
t th
e
c
r
u
d
e
b
r
o
m
elain
.
2
.
2
.
2
.
M
a
nu
f
a
ct
uring
o
f
bla
c
k
s
o
y
m
is
o
T
h
e
m
an
u
f
ac
t
u
r
in
g
p
r
o
ce
s
s
o
f
m
is
o
r
ef
er
s
to
[
1
7
]
with
m
o
d
if
icatio
n
.
B
lack
s
o
y
b
ea
n
s
wer
e
wash
ed
f
ir
s
t
b
ef
o
r
e
b
ein
g
m
ash
e
d
an
d
s
o
ak
ed
with
wate
r
f
o
r
1
2
h
o
u
r
s
.
T
o
m
ak
e
a
s
ter
ilized
b
lack
s
o
y
b
ea
n
,
b
o
il
it
f
o
r
2
0
m
in
u
tes
at
±
1
0
0
º
C
.
T
h
e
b
o
iled
b
lack
s
o
y
b
ea
n
was
th
en
f
ilter
ed
to
s
ep
a
r
ate
th
e
wate
r
an
d
its
s
o
lid
s
.
T
h
e
b
lack
s
o
y
b
ea
n
s
wer
e
d
iv
id
ed
i
n
to
f
iv
e
p
ar
ts
,
with
ea
ch
p
ar
t w
eig
h
in
g
1
5
0
g
o
f
wet
b
asis
.
E
ac
h
s
am
p
le
o
f
m
is
o
was
m
ix
ed
with
cr
u
d
e
b
r
o
m
elain
ac
co
r
d
in
g
to
th
e
t
r
ea
tm
en
t
(
w/w)
n
am
ely
0
%
(
T
0
)
,
3
%
(
T
1
)
,
6
%
(
T
2
)
,
9
%
(
T
3
)
,
an
d
1
2
%
(
T
4
)
an
d
r
est
f
o
r
3
h
o
u
r
s
th
en
ad
d
ed
with
2
5
%
(
w/w)
b
lack
s
o
y
b
ea
n
k
o
ji,
1
3
%
(
w/w)
s
alt,
4
%
(
w/w)
s
u
g
ar
,
an
d
4
%
(
w/w)
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
s
u
s
p
en
s
io
n
.
T
h
e
m
i
x
tu
r
e
was
s
tirr
ed
u
n
til
d
is
tr
ib
u
ted
,
co
m
p
ac
ted
in
a
p
last
ic
co
n
tain
er
,
an
d
f
er
m
en
te
d
at
r
o
o
m
tem
p
er
atu
r
e
f
o
r
7
d
ay
s
.
2
.
2
.
3
.
P
a
ra
m
et
er
s
t
esting
T
h
e
p
ar
am
ete
r
ass
ess
m
en
t
was
ca
r
r
ied
o
u
t
o
n
b
lack
s
o
y
b
ea
n
m
is
o
tr
ea
ted
with
c
r
u
d
e
b
r
o
m
elain
(
w/w)
0
%
(
T
0
)
as
a
co
n
tr
o
l,
3
%
(
T
1
)
,
6
%
(
T
2
)
,
9
%
(
T
3
)
,
a
n
d
1
2
%
(
T
4
)
.
T
h
e
wate
r
co
n
t
en
t
test
in
g
u
s
e
s
th
e
o
v
en
d
r
y
in
g
m
eth
o
d
b
y
weig
h
in
g
th
e
m
is
o
an
d
p
u
t
tin
g
it
i
n
th
e
o
v
en
f
o
r
8
h
o
u
r
s
[
1
8
]
.
T
h
e
wate
r
ac
tiv
ity
test
was
ca
r
r
ied
o
u
t
u
s
in
g
a
ca
lib
r
ated
a
w
m
eter
.
T
h
e
p
H
test
in
g
was
ca
r
r
ied
o
u
t
[
1
9
]
u
s
in
g
a
p
H
m
eter
.
L
AB
v
iab
ilit
y
was c
ar
r
ied
o
u
t t
o
[
2
0
]
u
s
in
g
th
e
p
o
u
r
p
late
m
et
h
o
d
with
1
0
-
7
d
ilu
tio
n
.
Evaluation Warning : The document was created with Spire.PDF for Python.
I
SS
N
:
2
2
5
2
-
8
8
1
4
I
n
t J Ad
v
Ap
p
l Sci
,
Vo
l.
1
3
,
No
.
4
,
Dec
em
b
er
2
0
2
4
:
818
-
82
4
820
2.
3
.
Da
t
a
a
na
ly
s
is
T
h
e
d
ata
w
er
e
an
aly
ze
d
u
s
in
g
an
ap
p
licatio
n
n
a
m
ed
SPSS
2
6
.
0
f
o
r
W
in
d
o
ws
.
T
h
e
d
ata
wa
s
o
b
tain
ed
by
test
in
g
t
h
e
wate
r
co
n
ten
t,
wate
r
ac
tiv
ity
,
an
d
L
AB
v
ia
b
ilit
y
.
I
t
was
an
al
y
ze
d
u
s
in
g
t
h
e
an
aly
s
is
o
f
v
a
r
ian
ce
(
ANOV
A)
p
ar
am
etr
ic
test
with
s
ig
n
if
ican
ce
lev
el
(
p
<0
.
0
5
)
an
d
wh
e
n
th
er
e
is
a
s
ig
n
if
i
ca
n
t
d
if
f
er
e
n
ce
in
tr
ea
tm
en
t
will
b
e
c
o
n
tin
u
ed
b
y
Du
n
ca
n
’
s
m
u
ltip
le
r
an
g
e
test
(
DM
R
T
)
wh
ile
th
e
L
AB
v
iab
ilit
y
was
s
tatis
t
ically
an
aly
ze
d
u
s
in
g
E
x
ce
l a
p
p
licatio
n
.
3.
RE
SU
L
T
S AN
D
D
I
SCU
SS
I
O
N
3
.
1
.
B
la
c
k
s
o
y
m
is
o
’
s
chem
i
ca
l c
ha
ra
ct
er
is
t
ics
T
h
e
c
h
em
ical
c
h
ar
ac
ter
is
tics
o
f
b
lack
s
o
y
m
is
o
wer
e
wate
r
c
o
n
ten
t,
wate
r
ac
tiv
ity
,
an
d
p
H
v
alu
e
as
d
escr
ib
e
d
i
n
Fig
u
r
e
1
.
C
h
em
ic
al
ch
ar
ac
ter
is
tics
o
f
b
lack
s
o
y
m
is
o
wer
e
wate
r
co
n
ten
t,
wat
er
ac
tiv
ity
,
an
d
p
H
v
alu
e
as
d
escr
ib
e
d
i
n
Fig
u
r
e
1
ca
n
b
e
s
ee
n
th
at
th
e
ad
d
itio
n
o
f
cr
u
d
e
b
r
o
m
elain
with
co
n
ce
n
tr
atio
n
s
o
f
0
,
3
,
6
,
9
,
an
d
1
2
%
g
iv
e
s
a
s
ig
n
if
ican
t
ef
f
ec
t (
p
<0
.
05)
.
T
h
e
av
er
a
g
e
wate
r
co
n
ten
t
o
f
b
lack
s
o
y
b
ea
n
m
is
o
in
th
is
s
tu
d
y
r
an
g
ed
f
r
o
m
5
6
.
92
-
61
.
0
0
%.
Miso
tr
ea
tm
en
t
with
th
e
ad
d
it
io
n
o
f
0
%
cr
u
d
e
b
r
o
m
elain
o
r
ac
tin
g
as
a
co
n
tr
o
l
h
as
th
e
s
m
alles
t
a
w
v
alu
e
wh
ic
h
is
0
.
6
5
3
w
h
ile
th
e
h
ig
h
est
a
w
v
alu
e
o
f
th
e
tr
ea
tm
en
t
is
m
is
o
with
th
e
ad
d
itio
n
o
f
9
%
cr
u
d
e
b
r
o
m
elain
wh
ich
is
0
.
6
7
1
.
T
h
e
h
ig
h
est p
H
v
alu
e
is
o
wn
ed
b
y
m
is
o
with
th
e
ad
d
itio
n
o
f
0
%
cr
u
d
e
b
r
o
m
elain
wh
ich
t
h
e
v
alu
e
is
5
.
1
0
an
d
th
e
lo
west
p
H
v
alu
e
is
o
wn
ed
b
y
m
is
o
with
th
e
ad
d
itio
n
o
f
1
2
%
cr
u
d
e
b
r
o
m
elain
wh
ic
h
th
e
v
al
u
e
is
4
.
70.
Fig
u
r
e
1
.
C
h
em
ical
ch
a
r
ac
ter
is
tic
s
o
f
b
lack
s
o
y
m
is
o
Fig
u
r
e
1
s
h
o
ws
th
at
th
e
ad
d
iti
o
n
o
f
c
r
u
d
e
b
r
o
m
elain
ca
n
in
c
r
ea
s
e
th
e
wate
r
co
n
te
n
t
o
f
b
lack
s
o
y
b
ea
n
m
is
o
.
T
h
e
h
ig
h
-
wate
r
co
n
ten
t
is
th
o
u
g
h
t
to
b
e
ca
u
s
ed
b
y
th
e
am
y
lo
ly
tic
ac
tiv
ity
o
f
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
.
Am
y
lo
ly
tic
ac
tiv
ity
b
y
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
ca
n
h
y
d
r
o
ly
ze
s
tar
ch
an
d
s
im
p
le
p
r
o
tein
s
to
p
r
o
d
u
ce
g
lu
co
s
e
an
d
o
th
er
s
u
g
a
r
s
wh
ich
ar
e
th
en
co
n
v
er
te
d
in
to
p
y
r
u
v
ate
b
y
f
r
ee
in
g
wate
r
m
o
lecu
les
[
1
0
]
s
o
th
at
th
e
am
o
u
n
t
o
f
wate
r
co
n
tai
n
ed
will a
ls
o
b
e
m
o
r
e.
An
o
th
er
f
ac
to
r
th
at
in
f
lu
e
n
ce
s
wate
r
co
n
ten
t
is
s
a
lt.
Acc
o
r
d
in
g
to
T
u
h
u
m
u
r
y
[
2
1
]
,
th
e
r
e
d
u
ce
d
wate
r
co
n
ten
t
o
f
a
p
r
o
d
u
ct
ca
n
b
e
ca
u
s
ed
b
y
th
e
p
r
esen
ce
o
f
s
a
lt
wh
ich
ca
n
r
ed
u
ce
th
e
wate
r
ac
tiv
ity
.
Salt
i
s
h
y
d
r
o
p
h
ilic
wh
ich
h
as
th
e
a
b
ilit
y
to
ab
s
o
r
b
wate
r
.
Salt
h
as
a
h
ig
h
er
o
s
m
o
tic
p
r
ess
u
r
e
b
ec
au
s
e
o
f
th
is
d
if
f
er
en
ce
in
p
r
ess
u
r
e
s
o
th
e
s
alt
will a
b
s
o
r
b
th
e
wate
r
in
th
e
m
ater
ial
u
n
til th
er
e
is
a
b
alan
ce
b
etwe
en
th
e
two
,
th
e
wate
r
th
at
co
n
tin
u
es to
b
e
ab
s
o
r
b
ed
will a
f
f
ec
t th
e
d
ec
r
ea
s
e
i
n
th
e
wate
r
co
n
ten
t v
al
u
e
o
f
th
e
m
ater
ial
[
2
2
]
.
Ho
wev
er
,
in
th
is
s
tu
d
y
,
s
alt
h
ad
n
o
ef
f
ec
t
o
n
th
e
wate
r
co
n
te
n
t
o
f
b
lack
s
o
y
b
ea
n
m
is
o
b
ec
a
u
s
e
th
e
co
n
ce
n
tr
atio
n
u
s
ed
was th
e
s
a
m
e.
T
h
e
h
i
g
h
er
ad
d
i
n
g
cr
u
d
e
b
r
o
m
elain
co
n
ce
n
tr
atio
n
g
iv
e
n
t
o
th
e
m
is
o
p
r
o
d
u
ct,
th
e
h
i
g
h
er
th
e
wate
r
ac
tiv
ity
v
alu
e
co
n
tain
e
d
.
T
h
e
a
w
v
alu
e
o
f
m
is
o
tr
ea
tm
en
t
h
a
d
a
m
ea
n
r
a
n
g
e
b
etwe
en
0
.
6
5
3
-
0
.
6
7
1
.
W
h
en
it
is
co
m
p
ar
ed
,
th
e
wate
r
ac
tiv
ity
v
alu
e
o
f
tr
ea
ted
m
is
o
is
lo
we
r
th
an
m
is
o
with
lo
w
s
al
t
co
n
ten
t.
Acc
o
r
d
in
g
to
Allwo
o
d
et
a
l.
[
2
3
]
,
m
is
o
with
a
lo
w
s
alt
co
n
ten
t
o
f
2
.
3
6
-
5
%
h
as
a
wate
r
ac
tiv
ity
v
alu
e
o
f
0
.
835
-
0
.
8
7
5
.
T
h
e
lo
w
o
f
a
w
v
alu
e
o
n
th
e
m
is
o
s
am
p
le
is
in
f
lu
en
ce
d
b
y
th
e
ad
d
ed
s
alt
co
n
ten
t.
Salt
it
s
e
lf
h
as
h
y
g
r
o
s
co
p
i
c
p
r
o
p
er
ties
wh
ich
can
b
in
d
wat
er
.
T
h
is
is
in
l
in
e
with
W
an
g
et
a
l.
[
2
4
]
wh
ich
s
tates
th
at
s
a
lt
h
as
th
e
ab
ilit
y
to
attr
ac
t
wate
r
m
o
lecu
les
s
o
t
h
at
wate
r
ac
tiv
ity
lev
els
d
ec
r
ea
s
e
an
d
ca
n
in
h
ib
it
th
e
g
r
o
wth
o
f
p
ath
o
g
en
ic
m
icr
o
b
es in
th
e
p
r
o
d
u
ct.
T
h
e
a
d
d
itio
n
o
f
c
r
u
d
e
b
r
o
m
ela
in
an
d
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
ca
n
s
p
ee
d
u
p
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
b
y
b
r
ea
k
in
g
d
o
wn
p
r
o
tein
s
in
t
o
s
im
p
ler
co
m
p
o
u
n
d
s
,
n
am
ely
am
in
o
ac
id
s
.
T
h
e
f
er
m
e
n
tatio
n
p
r
o
ce
s
s
is
clo
s
ely
5
.
1
4
.
9
3
4
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8
8
4
.
7
9
4
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7
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5
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6
5
6
0
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6
5
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6
7
1
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.
6
6
1
5
6
.
9
3
5
7
.
6
5
5
8
.
9
5
9
.
4
61
54
56
58
60
62
0
2
4
6
T0
T1
T2
T3
T4
W
ater
C
o
n
ten
t
(
%)
p
H
&
W
ater
A
ctiv
ity
Valu
e
Ch
e
m
ic
a
l
Ch
a
r
a
c
te
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isti
c
o
f
B
la
c
k
so
y
M
iso
p
H
V
a
lu
e
a
w Va
lu
e
Wate
r
Co
n
ten
t
Evaluation Warning : The document was created with Spire.PDF for Python.
I
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t J Ad
v
Ap
p
l Sci
I
SS
N:
2252
-
8
8
1
4
C
h
a
r
a
cteris
tic
o
f b
la
ck
s
o
y
mi
s
o
w
ith
cru
d
e
b
r
o
mela
in
a
n
d
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
…
(
B
h
a
kti
E
tz
a
S
etia
n
i
)
821
r
elate
d
to
L
AB
.
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
is
a
ty
p
e
o
f
L
AB
t
h
at
ca
n
f
er
m
en
t
s
u
g
ar
in
to
lact
ic
ac
id
an
d
is
o
f
te
n
fo
u
n
d
in
f
er
m
e
n
ted
f
o
o
d
p
r
o
d
u
cts
s
u
ch
as
m
is
o
.
Su
g
ar
is
u
s
ed
b
y
L
AB
as
a
s
u
b
s
tr
ate
o
r
s
o
u
r
ce
o
f
n
u
tr
ien
ts
to
d
ev
elo
p
.
T
h
is
is
in
lin
e
with
L
iu
et
a
l.
[
2
5
]
wh
ich
s
tates
th
at
g
lu
co
s
e
an
d
s
u
cr
o
s
e
f
u
n
ctio
n
as
ca
r
b
o
n
f
o
r
L
AB
f
er
m
en
tatio
n
an
d
will
d
ec
r
ea
s
e
alo
n
g
with
L
AB
g
r
o
wth
an
d
m
etab
o
lis
m
.
T
h
e
f
er
m
e
n
tatio
n
p
r
o
ce
s
s
will
ca
u
s
e
th
e
m
etab
o
lis
m
o
f
L
AB
.
T
h
e
in
cr
ea
s
e
in
wate
r
ac
tiv
ity
in
m
is
o
ca
n
also
b
e
d
u
e
to
th
e
a
m
y
lo
ly
tic
ac
tiv
ity
o
f
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
b
ec
a
u
s
e
it
u
s
es
s
tar
ch
as
a
s
u
b
s
tr
at
e.
Am
y
lo
ly
tic
ac
tiv
ity
in
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
ca
n
h
y
d
r
o
ly
ze
s
tar
ch
a
n
d
p
r
o
tein
in
to
s
im
p
le
s
u
g
ar
s
an
d
will
b
e
co
n
v
er
ted
in
to
p
y
r
u
v
ate
b
y
f
r
ee
in
g
wate
r
m
o
lecu
les
s
o
th
at
th
e
h
y
d
r
o
x
y
l
g
r
o
u
p
s
in
th
e
s
tar
ch
g
r
an
u
les
d
is
ap
p
ea
r
an
d
th
e
wate
r
co
n
te
n
t
in
th
e
p
r
o
d
u
ct
is
g
r
ea
ter
.
T
h
e
p
H
v
alu
e
is
an
i
n
d
icato
r
to
d
eter
m
in
e
t
h
e
ac
id
ity
lev
el
o
f
a
p
r
o
d
u
ct.
T
h
e
am
o
u
n
t
o
f
H
+
io
n
co
n
ce
n
tr
atio
n
co
n
tain
e
d
in
th
e
s
o
lu
tio
n
b
ec
o
m
es
a
r
ef
er
e
n
ce
in
d
eter
m
in
i
n
g
th
e
p
H
v
alu
e
.
T
h
e
p
H
v
alu
e
will
d
ec
r
ea
s
e
o
r
b
ec
o
m
e
m
o
r
e
ac
id
ic
as
th
e
cr
u
d
e
b
r
o
m
elain
co
n
c
en
tr
atio
n
in
c
r
ea
s
es.
Miso
with
th
e
ad
d
itio
n
c
r
u
d
e
b
r
o
m
elain
co
n
ce
n
tr
atio
n
o
f
1
2
%
s
h
o
wed
a
lo
wer
p
H
v
alu
e
co
m
p
a
r
ed
to
o
th
er
tr
ea
tm
e
n
ts
.
Miso
with
th
e
ad
d
itio
n
o
f
d
if
f
er
e
n
t
c
o
n
ce
n
tr
a
tio
n
s
o
f
cr
u
d
e
b
r
o
m
elain
will
p
r
o
d
u
ce
d
i
f
f
er
en
t
p
H
v
alu
es.
T
h
e
h
ig
h
er
o
f
cr
u
d
e
b
r
o
m
elain
c
o
n
ce
n
tr
atio
n
ad
d
e
d
,
th
e
ac
id
ity
le
v
el
ten
d
s
to
in
c
r
ea
s
e.
C
r
u
d
e
b
r
o
m
elain
is
class
if
ied
as
a
p
r
o
tease
en
zy
m
e
th
at
is
a
b
le
to
s
p
ee
d
u
p
th
e
f
er
m
en
tati
o
n
p
r
o
ce
s
s
b
y
b
r
ea
k
in
g
d
o
wn
p
r
o
tein
s
in
t
o
s
im
p
ler
co
m
p
o
u
n
d
s
,
n
am
ely
am
in
o
ac
id
s
.
T
h
e
ty
p
es
o
f
a
m
in
o
ac
i
d
s
th
at
a
r
e
q
u
ite
o
f
ten
f
o
u
n
d
i
n
f
er
m
en
ted
p
r
o
d
u
cts
ar
e
n
o
n
-
ess
en
tial
am
in
o
ac
id
s
s
u
ch
as
g
l
u
tam
ic
ac
i
d
an
d
asp
a
r
tic
a
cid
d
u
e
to
th
e
ab
u
n
d
a
n
t
am
o
u
n
t
o
f
p
r
o
tei
n
[
2
5
]
.
T
h
ese
two
ty
p
es
o
f
am
in
o
ac
id
s
a
r
e
w
h
at
m
ak
e
it
p
o
s
s
ib
le
to
r
ed
u
ce
th
e
p
H
v
alu
e
o
f
th
e
p
r
o
d
u
ct
b
ec
au
s
e
th
ey
ar
e
class
if
ied
as
ac
id
ic
am
in
o
ac
id
s
.
T
h
i
s
is
in
lin
e
with
th
e
o
p
in
io
n
o
f
Ag
iu
s
et
a
l.
[
2
6
]
wh
ic
h
s
tates
th
at
g
lu
tam
ic
ac
id
an
d
asp
ar
tic
ac
id
ar
e
th
e
o
n
ly
p
r
o
tein
o
g
e
n
ic
am
in
o
ac
id
s
with
ac
id
ic
s
id
e
ch
ain
s
b
ec
au
s
e
th
ese
s
id
e
ch
ain
s
ar
e
ch
ar
g
e
d
an
d
h
y
d
r
o
p
h
ilic
an
d
ar
e
o
f
te
n
f
o
u
n
d
o
n
th
e
s
u
r
f
ac
e
o
f
p
r
o
tei
n
s
.
T
h
e
h
ig
h
L
AB
co
n
ten
t
in
b
la
ck
s
o
y
b
ea
n
m
is
o
k
o
ji
is
d
u
e
t
o
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
its
elf
an
d
th
e
ad
d
itio
n
s
o
f
La
cto
b
a
cillu
s
p
la
n
ta
r
u
m
T
h
is
also
aim
s
to
s
p
ee
d
u
p
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
.
Hak
im
et
a
l
.
[
2
7
]
s
tated
th
at
th
e
L
A
B
m
etab
o
lite
p
r
o
ce
s
s
d
u
r
in
g
th
e
f
er
m
e
n
tatio
n
p
r
o
ce
s
s
will
p
r
o
d
u
ce
lactic
ac
id
an
d
o
th
er
o
r
g
an
ic
ac
id
s
s
o
t
h
at
it
ca
n
r
ed
u
ce
th
e
p
H
v
alu
e
o
f
f
e
r
m
e
n
ted
s
o
y
b
ea
n
p
aste
p
r
o
d
u
cts.
I
n
th
e
f
er
m
en
tatio
n
p
r
o
ce
s
s
,
th
er
e
will
b
e
a
b
r
ea
k
d
o
wn
o
f
g
lu
co
s
e
in
to
lactic
ac
id
wh
ich
is
f
o
llo
wed
b
y
an
in
cr
ea
s
e
in
t
h
e
co
n
ce
n
tr
atio
n
o
f
H
+
wh
ich
ca
n
lo
we
r
th
e
p
H.
3
.
2
.
B
la
c
k
s
o
y
m
is
o
’
s
m
icro
bia
l c
ha
ra
ct
er
is
t
ic
T
h
e
m
icr
o
b
ial
ch
ar
ac
ter
is
tic
o
f
b
lack
s
o
y
m
is
o
is
th
e
L
A
B
v
iab
ilit
y
as
d
escr
ib
e
d
i
n
Fi
g
u
r
e
2
.
T
h
e
m
icr
o
b
ial
c
h
ar
ac
ter
is
tic
o
f
b
la
c
k
s
o
y
m
is
o
s
h
o
ws
a
wav
i
n
g
L
AB
g
r
o
wth
as
d
escr
ib
e
d
i
n
Fig
u
r
e
2
b
ec
a
u
s
e
of
th
e
ad
d
itio
n
o
f
cr
u
d
e
b
r
o
m
elai
n
with
d
if
f
er
e
n
t
c
o
n
ce
n
tr
atio
n
s
.
L
AB
g
r
o
wth
ex
p
er
ien
ce
d
it
s
o
p
tim
al
p
h
ase
o
n
th
e
3
rd
d
ay
o
f
f
er
m
en
tatio
n
,
it
r
ea
ch
ed
9
.
1
1
lo
g
C
FU/m
L
in
m
is
o
with
th
e
ad
d
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o
f
3
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u
d
e
b
r
o
m
elain
.
On
th
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7
th
d
a
y
o
f
f
e
r
m
en
tatio
n
,
L
AB
g
r
o
wth
d
ec
r
ea
s
es
u
n
til
8
.
3
5
lo
g
C
FU/m
L
in
m
is
o
with
t
h
e
ad
d
itio
n
o
f
cr
u
d
e
b
r
o
m
elain
o
f
3
%
as
well.
On
t
h
e
1
0
th
d
ay
o
f
f
e
r
m
en
tatio
n
ac
tu
ally
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an
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cr
ea
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e
in
L
AB
g
r
o
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ea
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ts
an
d
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o
m
e
g
r
o
wth
w
as st
ab
le
o
r
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en
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r
ea
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ed
.
Fig
u
r
e
2
.
Mic
r
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b
ial
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ar
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7
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Evaluation Warning : The document was created with Spire.PDF for Python.
I
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:
2
2
5
2
-
8
8
1
4
I
n
t J Ad
v
Ap
p
l Sci
,
Vo
l.
1
3
,
No
.
4
,
Dec
em
b
er
2
0
2
4
:
818
-
82
4
822
T
h
e
n
u
m
b
er
o
f
L
AB
g
r
o
wth
o
n
f
er
m
en
tatio
n
d
ay
0
is
s
till
s
m
all
b
ec
au
s
e
th
e
b
ac
ter
ia
n
ee
d
to
ad
ap
t to
th
eir
n
ew
en
v
ir
o
n
m
en
t.
T
h
is
is
in
ac
co
r
d
an
ce
with
Nico
la
an
d
B
aa
th
[
2
8
]
w
h
ich
s
tates
th
at
o
n
d
ay
0
o
f
f
er
m
en
tatio
n
,
La
ct
o
b
a
cillu
s
s
p
.
is
in
th
e
lag
p
h
ase
s
o
th
eir
g
r
o
wth
is
n
o
t
o
p
tim
al
b
ec
a
u
s
e
th
e
b
ac
ter
ia
ar
e
ca
r
r
y
in
g
o
u
t
an
ac
clim
atiza
tio
n
p
r
o
ce
s
s
to
en
v
ir
o
n
m
en
tal
co
n
d
itio
n
s
,
in
th
is
ca
s
e
,
ar
e
p
H
v
alu
e,
tem
p
er
atu
r
e,
an
d
n
u
tr
itio
n
.
E
n
te
r
in
g
th
e
3
rd
d
ay
o
f
f
er
m
en
tatio
n
,
L
AB
g
r
o
wth
in
cr
ea
s
e
d
in
all
tr
ea
tm
en
t
s
b
ec
au
s
e
L
AB
h
a
d
b
eg
u
n
to
g
r
o
w
an
d
en
ter
e
d
th
e
ex
p
o
n
en
tial p
h
ase
wh
er
e
b
ac
t
er
ial
g
r
o
wth
o
cc
u
r
r
ed
v
er
y
q
u
i
ck
ly
.
Acc
o
r
d
in
g
t
o
Sh
a
et
a
l.
[
2
9
]
s
tated
th
at
in
th
is
ex
p
o
n
en
tial
p
h
ase
,
m
icr
o
b
es
ar
e
ca
r
r
y
in
g
o
u
t
ce
ll
d
iv
is
io
n
o
r
m
u
ltip
licatio
n
.
T
h
e
g
r
ea
ter
t
h
e
cr
u
d
e
b
r
o
m
el
ain
co
n
ce
n
tr
atio
n
ad
d
ed
,
th
e
f
aster
th
e
g
r
o
wth
o
f
L
AB
.
T
h
is
is
in
ac
co
r
d
an
ce
with
Utam
i
et
a
l.
[
3
0
]
wh
o
s
tated
th
at
th
e
b
r
o
m
elain
e
n
zy
m
e
is
a
p
r
o
tease
en
zy
m
e
th
at
ca
n
ac
ce
ler
ate
th
e
co
n
v
er
s
io
n
o
f
p
r
o
tein
b
o
n
d
s
in
to
am
in
o
ac
id
s
wh
o
s
e
n
itro
g
e
n
co
n
ten
t
p
lay
s
a
r
o
le
in
th
e
f
o
r
m
atio
n
o
f
b
i
o
m
ass
ce
ll
s
in
L
AB
g
r
o
wth
.
T
h
e
f
ast
er
th
e
f
er
m
e
n
tatio
n
p
r
o
ce
s
s
,
th
e
m
o
r
e
L
AB
g
r
o
wth
will in
cr
e
ase.
L
AB
g
r
o
wth
b
eg
a
n
to
d
ec
lin
e
o
n
th
e
7
th
d
ay
o
f
f
er
m
en
tatio
n
b
ec
au
s
e
it
was
ap
p
r
o
ac
h
i
n
g
its
d
ea
th
p
h
ase
af
ter
en
ter
in
g
th
e
s
tatio
n
ar
y
p
h
ase
b
etwe
en
th
e
3
rd
an
d
7
th
d
ay
s
.
B
ac
ter
ial
g
r
o
wth
in
th
e
s
tatio
n
ar
y
p
h
ase
is
r
elativ
ely
co
n
s
tan
t
b
ec
au
s
e
th
e
n
u
m
b
er
o
f
ce
lls
th
at
g
r
o
w
is
th
e
s
am
e
as
th
e
n
u
m
b
er
o
f
ce
lls
th
at
d
ie,
a
n
d
ev
en
r
elativ
ely
d
ec
r
ea
s
es b
ec
a
u
s
e
th
e
n
u
tr
ien
ts
in
th
e
m
ed
ia
b
eg
in
to
d
ec
r
ea
s
e
[
3
1
]
.
T
h
is
is
co
n
s
is
ten
t w
ith
th
e
10
th
d
ay
o
f
f
er
m
en
tatio
n
s
h
o
win
g
th
at
L
AB
g
r
o
wth
h
as
in
cr
ea
s
ed
ag
ain
in
s
ev
er
al
tr
ea
tm
en
ts
,
is
s
tab
le,
o
r
ev
en
d
ec
r
ea
s
ed
.
T
h
e
in
cr
ea
s
e
in
L
AB
g
r
o
wth
m
ea
n
s
th
at
th
e
b
ac
ter
ia
ar
e
s
till
in
th
e
ex
p
o
n
en
tial
p
h
ase.
A
s
tab
le
n
u
m
b
er
o
f
L
AB
co
lo
n
ie
s
in
d
icate
s
th
at
th
e
b
ac
ter
ia
ar
e
in
th
e
s
tatio
n
ar
y
p
h
ase.
4.
CO
NCLU
SI
O
N
T
h
e
ad
d
itio
n
o
f
cr
u
d
e
b
r
o
m
elain
in
m
is
o
m
ad
e
f
r
o
m
b
lack
s
o
y
b
ea
n
s
g
iv
es
d
if
f
er
e
n
t
r
esu
l
ts
in
ea
ch
p
ar
am
eter
.
T
h
e
a
d
d
itio
n
o
f
cr
u
d
e
b
r
o
m
elain
in
m
is
o
ca
n
r
e
d
u
ce
th
e
p
H
v
alu
e,
a
n
d
i
n
cr
ea
s
e
th
e
wate
r
co
n
ten
t
an
d
a
w
v
alu
e,
b
u
t h
as
n
o
t p
r
o
v
id
ed
o
p
tim
al
r
esu
lts
f
r
o
m
a
m
i
cr
o
b
io
lo
g
ical
asp
ec
t.
T
h
e
b
est tr
ea
tm
en
t
f
r
o
m
th
e
r
esear
ch
co
n
d
u
cted
is
m
is
o
with
ad
d
itio
n
s
o
f
cr
u
d
e
b
r
o
m
elain
en
zy
m
e
9
%
b
ec
au
s
e
it
is
v
er
y
clo
s
e
to
t
h
e
d
esire
d
cr
iter
ia
wh
ich
ar
e
h
av
i
n
g
a
h
i
g
h
-
wate
r
c
o
n
ten
t,
a
w
v
a
lu
e
h
ig
h
,
an
d
l
o
w
p
H
v
alu
es.
ACK
NO
WL
E
DG
E
M
E
NT
S
T
h
e
a
u
th
o
r
th
an
k
s
to
I
n
d
o
f
o
o
d
R
is
et
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g
r
ah
a
wh
o
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in
an
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t
with
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e
f
u
n
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u
m
b
er
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ch
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o
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o
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atio
n
ag
r
e
em
en
t SKE
.
0
2
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2
.
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F
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R
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NC
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S
[
1
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S
.
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n
g
,
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.
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u
,
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a
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,
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o
,
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.
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h
a
n
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,
a
n
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Y
.
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h
e
n
,
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f
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t
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p
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d
s
o
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t
h
e
q
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a
l
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y
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f
m
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s
o
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t
r
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1
3
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p
p
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2
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a
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.
,
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